高效液相色谱-蒸发光散射检测法测定全营养混合液中黄芪甲苷含量

目的建立测定全营养混合液（TNA）中黄芪甲苷含量的方法。方法采用高效液相色谱-蒸发光散射检测（HPLC—ELSD）法对TNA中黄芪甲苷的含量进行测定，色谱柱为Kmmasil C18柱（250mm×4．6mm，5μm），流动相为乙腈-水（35：65），流速为1．0mL／min，ELSD参数为漂移管温度105℃，气体流速2．8L／min。结果黄芪甲苷对照品进样量在2．10～10．50μg范围内与峰面积线性关系良好（r=0．9952），平均回收率为96．96％，RSD=1．69％（n=9）。结论HPLC—ELSD法操作简便、结果准确、重现性好，可作为该制剂中黄芪甲苷含量的检测方法。

Determination of Astragaloside IV in Total Nutrient Admixture by HPLC-ELSD

Objective To establish the assaying method of astragaloside Ⅳ in total nutrient admixture （TNA）. Methods To determine astragaloside IV in TNA by HPLC- ELSD. Kromasil C18 column （250 mm ×4. 6 mm, 5 μm） was used. The mobile phase was acetonitrile- water （35：65）.The flow rate was at 1.0 mL/min. The drift tube temperature was at 105 ℃ and the gas flow rate was 2.8 L/min. Results A good linear relationship between the content and peak area of astragaloside IV was found within the range of 2. 10- 10. 50 μg. The regression coefficient was 0. 995 2 and the average recovery rate was 96.96%, RSD was 1.69% （n =9）. Conclusion The method is convenient, accurate with fine reproducibility. It can be used for the quality control of astragaloside Ⅳ.