垂体肿瘤转化因子1和Ten-eleven转运因子1在肝细胞癌中的表达及相关性分析

目的探讨肝癌组织中垂体肿瘤转化因子1(PTTG1)及Ten-eleven转运因子1(TET1)表达水平及其与临床病理的相关性,为预测肝癌的发生及发展提供理论基础。方法选择2017年2月至2019年1月恩施土家族苗族自治州中心医院行根治性切除手术肝细胞癌病例标本129例作为实验组,另选取129例对应的癌旁组织作为对照组。应用免疫组织化学法检测PTTG1和TET1在肝癌组织及癌旁组织中的表达,分析PTTG1和TET1的表达与肝细胞癌临床病理指标的关系,对PTTG1和TET1表达水平进行相关性分析。结果PTTG1在肝细胞癌组织的高表达率(53.5%,69/129)显著高于癌旁组织(1.6%,2/129)(χ^2=84.646,P<0.001),TET1在癌组织的表达(45.0%,58/129)显著低于癌旁组织(81.4%,105/129)(χ^2=35.255,P<0.001)。PTTG1的表达水平与TET1表达水平呈负相关关系(r=-0.726,P<0.001)。PTTG1的表达水平与卫星结节、Edmondson病理分级密切相关(P<0.05),TET1的表达水平与肿瘤包膜状况、卫星结节密切相关(P<0.05)。结论PTTG1和TET1在肝细胞癌组织中异常表达,并且与肝细胞癌的发生、发展密切相关,临床上有望成为肝癌的诊断和根治性切除治疗预后的生物标志物。

Expression of PTTG1 and TET1 in hepatocarcinoma tissues and its relationship with clinicopathological parameters

Objective To investigate the correlation between the expression levels of pituitary tumor transforming gene 1(PTTG1)and ten-eleven translocation 1(TET1),and the clinicopathological parameters in liver cancer tissues and paracancer tissues,and to provide theoretical basis for the prediction of the occurrence and development of liver cancer.Methods From February 2017 to January 2019,129 cases of hepatocellular carcinoma specimens undergoing radical resection in Central Hospital of Enshi Tujia and Miao Autonomous Prefecture were selected as the experimental group,and 129 corresponding adjacent tissues were selected as the control group.Immunohistochemistry technique was used to detect the expression of PTTG1 and TET1 in the two groups.The relationship between the clinicopathological parameters and the expression of PTTG1 and TET1 was analyzed.In addition,the relationship between the PTTG1 protein and TET1 protein was correlation analyzed.Results The high expression rate of PTTG1 in hepatocellular carinoma tissues(53.5%,69/129)was significantly higher than that in adjacent tissues(1.6%,2/129)(χ^2=84.646,P<0.001).The expression of TET1 in hepatocellular carinoma tissues(45.0%,58/129)was significantly lower than that in adjacent tissues(81.4%,105/129)(χ^2=35.255,P<0.001).The expression level of PTTG1 was negatively correlated with that of TET1(r=-0.726,P<0.001).The expression level of PTTG1 was closely related to satellite nodules and Edmondson pathological grade(P<0.05),and the expression level of TET1 was closely related to the tumor envelope status and satellite nodules(P<0.05).Conclusion PTTG1 protein and TET1 protein are expressed abnormally in hepatocellular carcinoma tissues,and are closely correlated with the occurrence and development of hepatocellular carcinoma,which suggests PTTG1 protein and TET1 protein can be used as biological indicators for the diagnosis and prognosis of hepatocellular carcinoma.