体外培养重建角膜组织的技术探讨

目的：探讨体外重建角膜组织的三维培养技术。方法：原代培养角膜上皮，基质和内皮细胞，利用硫酸软骨素，透明质酸钠，I型胶原蛋白和微孔材料，建立三维培养系统，体外重建角膜组织结构，观察非醛交联，醛交联和微孔材料等三类人工角膜的透明度，常规病理组织学检测细胞在三维培养系统中生长状态。结果：非醛交联类重建人工角膜质地较软，透明度较低，光镜可见纤维结构，醛交联类重建人工角膜质地较韧，透明性较高，光镜可见均质结构，非吸收微孔材料类细胞长人较少，较难体外重建人工角膜，结论：利用生物工程技术能够体外重建人工角膜，非醛交联类结构较规则，醛交联类透明度较高。

Technology about Reconstruction of cornea Three-dimensional culture system in vitra

Objective: To research the technology about reconstruction of cornea three-dimensional culture system in Vitra. Methods: using primary cultured corneal epithelial cells, stoma cells and endothelial cells rebuilt corneal structure in three dimensional culture system by hyaluronate, chondroitin, type I collagen and microporous materials. To exam transparency of nonaldehyde-derived cross-link, aldehyde-derived cross-link and microporous materials type artificial corneal by slit microcopy. The biological characters of artificial cornea are tested by routine pathological method. Results: The non-aldehyde-derived corss-link type artificial corneal is soften in character, lower in transparency and its fiber structure is visible under microscope. The aldehyde-derived cross-link type artificial corneal is tougher in character, higher in transparency and there is a homogeneous structure under microscope. Few of keratocytes can grow in micropore in microporous materials. It cannot re-built an artificial corneal by microporous materials. Conclusions: the transparent corneal structure can be rebuilt by tissue engineering technology in vita. The non-aldehyde-derived cross-link type artificial cornea has more regular structure and the aldehyde-derived cross-link type has more transparent than others.