钬激光治疗兔肾VX2肿瘤的实验研究

目的探讨钬激光在治疗肾实质肿瘤中的应用。方法制作兔的肾VX2肿瘤模型，超声影像学技术初步评估肿瘤组织的大小，在手术直视下，应用不同的钬激光照射参数，对肿瘤组织进行照射。通过肉眼、光镜及图像处理软件。观察钬激光对肿瘤组织的生物学效应及范围。结果钬激光照射后肿瘤组织肉眼观察被完全凝固。分为汽化层、凝固坏死层、外围损伤层三层。HE染色可发现肿瘤细胞发生凝固性坏死，电镜下亦见到不可逆的破坏。切割带外围热损伤的宽度局限于0．4～0．8mm（平均0．54mm）。激光功率与损伤的肿瘤组织大小存在正相关关系。结论钬激光对肾VX2肿瘤组织具有精确的汽化、切割、凝固效果，同时止血可靠，可以通过调整激光的功率控制毁损肿瘤组织的范围，可操作性强。可以用于肾实质肿瘤的临床治疗，也可在影像技术的引导下行穿刺介入治疗，或者通过腹腔镜下治疗，以及应用于保肾手术中的辅助切割。

Treatment of Ho: YAG laser on VX2 renal tumor in rabbits

[Objective] To investigate the effectiveness of Ho:YAG laser irradiation in the treatment of kidney tumors.[Methods] Ten New Zealand rabbits bearing VX2 kidney tumors were irradiated by Ho:YAG laser with certain parameter in vivo.The vaporized incision,coagulation,haemostatic effect and pathologic alteration of laser to tumor tissues were observed and measured.[Results] Tumor cells underwent coagulation necrosis immediately after irradiation with Ho:YAG.There were three layers after Ho:YAG laser irradiation(The layer of vaporization,coagulation necrosis and thermal damage).The width of thermal damage and its controls were determined by computer image analysis.Tissues.There was a positive correlation between irradiation power and ablation effect.The control was easily handled.[Conclusion] The experiment indicates that Ho:YAG laser should be effective in the treatment of kidney tumors.