氡染毒大鼠肺组织病理改变与CcO活性的相关性研究

目的 观察氡染毒对大鼠肺组织的病理改变以及线粒体细胞色素C氧化酶(CcO)活性的影响。方法 多功能生态氡室对Wistar大鼠进行氡及其子体染毒,累积染毒剂量分别达100、200、400WLM,染毒后观察各组大鼠的肺组织损伤程度,提取大鼠肺组织线粒体进行CcO活性的测定。结果 染毒后肺组织主要病理变化为肺间质充血水肿,炎症细胞浸润,纤维化变性,肺泡腔萎缩消失。线粒体CcO的活性随着染氡剂量的增加逐渐降低,并且与肺泡间隔厚度成负相关,与肺泡腔面积成正相关。结论 氡染毒后肺组织的病理改变与CcO的活性相关。

The Correlative Study on the Pathological Alteration and Cytochrome c Oxidase Activity in Rat Lung Tissue Induced by Radon

Objective To observe the pathological change in rats exposed to radon, and to determine the cytochrome C oxidase activity in lung mitochondria.Methods Wistar rats were exposed to radon in a multifunction ecological radon chamber with the cumulative dose up to 100、200 and 400WLM respectively.The degree of lung injury was observed with HE staining and masson staining.The cytochrome C oxidase activity was determined by kit assay.Results In rats exposed to radon, a series of pathological alterations were observed including hyperaemia and edema in the alveolus interval, inflammatory cells such as lymphocytes were observed to penetrate mucous membrane and collagen fibre filled in interstitial of the alveolar.The enzyme activity was reduced in the mitochondria with the assay, and negative correlative to the thickness of alveolar septum, and positive correlative to the area of alveolar space.Conclusion The energy metabolism disorder may be induced by radon, Cytochrome C oxidase is probably one target of radon, and cell apoptosis in mitochondria maybe one of the pathways of radon biological effect.