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| Ald^1-Helicokinin Ⅰ的合成研究 | |
| 引用本文： | 陈道煌,陶炳志,黄照,陈河如,李丽芳.Ald^1-Helicokinin Ⅰ的合成研究[J].汕头大学医学院学报,2010,23(2):71-72. |
| 作者姓名： | 陈道煌 陶炳志 黄照 陈河如 李丽芳 |
| 作者单位： | 1. 汕头大学医学院化学教研室,广东,汕头,515041 2. 暨南大学中药及天然药物研究所 3. 汕头大学医学院化学教研室,广东,汕头,515041;暨南大学中药及天然药物研究所;暨南大学中药药效物质基础及创新药物研究所,广东省高校重点实验室,广东,广州,510632 |
| 基金项目： | 国家自然科学基金资助项目，广东省高等院校自然科学重点研究项目 |
| 摘 要： | 目的：用荧光非天然氨基酸Aladan（Ald）改造昆虫神经肽Helicokinin Ⅰ。方法：采用多肽固相合成法合成Ald1-Heli-cokinin Ⅰ,基质辅助激光解吸电离质谱确证,经反相高效液相色谱法纯化,纯度达97%以上。结果与结论：Ald1-Helicokinin Ⅰ的合成具有快捷、简便、高效等特点,最终收率31.8%,且Ald1-Helicokinin Ⅰ可作为荧光探针,进一步研究配体跟受体的相互作用。 |
| 关 键 词： | helicokinin Ⅰ 神经肽 固相多肽合成 |
Study on the Synthesis of Ald1-Helicokinin I |
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| CHEN Dao-huang,TAO Bing-zhi,HUANG Zhao,CHEN He-ru,LI Li-fang.Study on the Synthesis of Ald1-Helicokinin I[J].Journal of Shantou University Medical College,2010,23(2):71-72. | |
| Authors: | CHEN Dao-huang TAO Bing-zhi HUANG Zhao CHEN He-ru LI Li-fang |
| Institution: | 1 Department of Chemistry,Shantou University Medical College,Shantou 515041,China,2 Institute of Traditional Chinese Medicine and Natural Products,Jinan University,Guangzhou 510632,China,3 Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou 510632,China) |
| Abstract: | Objective： To modify the insect neuropeptide Helicolinin Ⅰ by fluorescent non-natural amino acid（Aladan,Ala）.Methods： Ald1-Helicokinin Ⅰ could be synthesized by solid-phase peptide synthesis,confirmed by matrix-assisted laser desorptionionization mass spectrometry,purified by revered-phase high performance liquid chromatography and the purity was up to 97%.Results and discussion： The synthesis method of Ald1-Helicokinin Ⅰ is very fast,simple and high-efficient,the final product rate is 31.8%.Ald1-Helicokinin Ⅰ can be used as fluorescence probe for the further investigation on the interaction between ligand and receptor. |
| Keywords: | helicokinin I |
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