Reactivity of purified lectins with blood typed canine erythrocytes

Agglutination tests were performed with 40 commercially available lectins and a panel of blood typed canine erythrocytes. Erythrocytes were obtained from 17 dogs (two poodles, two black labradors, five greyhounds and eight beagles). The erythrocytes expressed various combinations of dog erythrocyte antigens (DEA) 1.1, 1.2 or null, 4 and 7. Lectin reactivity with untreated, ficin treated, and neuraminidase-treated cells was determined. No correlation between lectin reactivity and the canine blood group antigens expressed on the red cell test panel was found. Results suggest that some canine erythrocytes may be differentiated from others on the basis of reactivity with Phytolacca americana and Glycine max lectins.