重组人类促红细胞生成素对早产鼠免疫功能影响的研究

目的　观察早产鼠应用重组人类促红细胞生成素 (rHuEPO)预防贫血的同时对其免疫功能的影响。方法　将早产鼠随机分为四组 :早产鼠低、高剂量促红细胞生成素 (EPO)组分别应用rHuEPO 2 5 0IU/(kg·次 )和 5 0 0IU/(kg·次 ) ,隔日 1次 ,连续 9次 ;早产鼠、足月鼠对照组应用等量的生理盐水。观察用药前后各组血红蛋白 (Hb)、血浆促红细胞生成素 (EPO)、红细胞免疫功能、T淋巴细胞增殖功能、细胞因子TNF α的变化。结果　早产鼠出生时红细胞免疫功能、T淋巴细胞增殖功能、细胞因子TNF α水平低于足月鼠。早产鼠生后Hb、红细胞免疫功能均逐渐下降 ,但应用rHuEPO的早产鼠下降幅度小 ,以高EPO组为著 ,与对照组相比差异有极显著意义 (P <0 0 1)。对照组早产鼠的C3b R %为 ( 11 0 0± 0 95 ) % ,高EPO组为 ( 17 75± 1 0 4) %。早产鼠生后T淋巴细胞增殖功能、TNF α均逐渐上升 ,但应用rHuEPO的早产鼠上升幅度大 ,以高EPO组为著 ,与对照组相比差异有极显著意义 (P均 <0 0 1)。对照组早产鼠T淋巴细胞增殖功能光密度 (A)值为 0 15 9± 0 0 14,高EPO组为0 3 5 4± 0 0 5 0。对照组早产鼠TNF α为 ( 0 2 70± 0 0 14)ng/ml,高EPO组为 ( 0 415± 0 0 10 )ng/ml。且T淋巴细胞增殖功能、TNF α与红细胞免疫功

Effects of recombinant human erythropoietin on the immune function of premature rats

OBJECTIVE: To observe the effect of recombinant human erythropoietin (rHuEPO) on immune function of premature rats. METHODS: RHuEPO of 250 IU/(kg.t) or 500 IU/(kg.t) was administered to premature rats every other day for nineteen days. The control premature rats were received normal saline. The changes of hemoglobin (Hb), serum erythropoietin (EPO), red blood cell (RBC) immune function, T lymphocyte proliferative responsiveness, and production of tumor necrosis factor alpha (TNF-alpha) were observed. RESULTS: Premature rats showed lower levels on Hb, RBC immune function, T cell responsiveness and production of TNF-alpha compared with mature rats at birth. The postnatal declines of Hb and RBC immune function were lessened in the treated groups of premature rats, the higher dosage group of 500 IU/(kg.t) was more significant than the lower dosage group of 250 IU/(kg.t). When experiments were over, Hb of control premature rats was (7.72 +/- 0.89) g/dl, Hb of premature rats received 500 IU/(kg.t) was (10.08 +/- 0.90) g/dl (P < 0.01). C3b-R% of control premature rats was (11.00 +/- 0.95)%, C3b-R% of premature rats received 500 IU/(kg.t) was (17.75 +/- 1.04)% (P < 0.01). IC-R% in control premature rats was (12.83 +/- 1.33)%, IC-R% of premature rats received 500 IU/(kg.t) was (10.50 +/- 1.67)% (P < 0.01). The postnatal rise of T cell responsiveness and the production of TNF-alpha in premature rats increased in the treated groups, which was more significant in the higher dosage group of 500 IU/(kg.t) than in the lower dosage group of 250 IU/(kg.t). The OD index of control premature rats was 0.159 +/- 0.014, the OD index of premature rats received 500 IU/(kg.t) was 0.354 +/- 0.050 (P < 0.01). TNF-alpha in control premature rats was (0.270 +/- 0.014) ng/ml, TNF-alpha of premature rats received 500 IU/(kg.t) was (0.415 +/- 0.010) ng/ml (P < 0.01). CONCLUSIONS: (1) Premature rats had lower RBC immune function and T cell responsiveness and underproduction of TNF-alpha at birth. (2) Premature rats had an improvement with the RBC immune function after rHuEPO administration. (3) Premature rats had improvements with T cell responsiveness and TNF-alpha after rHuEPO administration, and there was a positive correction between the RBC immune function and T cell responsiveness with the production of TNF-alpha.