| Immune Killing Activity of Lymphocytes on Hela Cells Expressing Interleukin-12 In Vitro |
| |
| 作者姓名： | 王慧燕 陈素华 |
| |
| 作者单位： | Hospital of Wuhan University of Technology，Department of Obstetrics and Gynecology Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430070，China，Wuhan 430030，China |
| |
| 摘 要： | The killing effects of lymphocytes on Hela cells expressing interleukin-12 （IL-12） in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT. It was found that Hela cells could express IL- 12 between 24 h and 72 h after transfection. Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced. It was concluded by cell transfection technique, Hela cells could express 1L-12 and were more easily killed by lymphocytes.
|
| 关 键 词： | 白介素-12 海拉细胞 免疫杀伤力活性 淋巴细胞 |
Immune killing activity of lymphocytes on Hela cells expressing interleukin-12 in vitro |
| |
| Huiyan Wang,Suhua Chen.Immune Killing Activity of Lymphocytes on Hela Cells Expressing Interleukin-12 In Vitro[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(3):343-345. |
| |
| Authors: | Huiyan Wang Suhua Chen |
| |
| Institution: | 1. Hospital of Wuhan University of Technology, Wuhan 430070, China
2. Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| |
| Abstract: | The killing effects of lymphocytes on Hela cells expressing interleukin-12(IL-12) in vitro were explored.By using gene transfection technique,full length IL-12 gene was transfected into Hela cells.The expression of IL-12 in Hela cells was detected quantitatively by ELISA;Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT.It was found that Hela cells could express IL-12 between 24 h and 72 h after transfection.Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced.It was concluded by cell transfection technique,Hela cells could express IL-12 and were more easily killed by lymphocytes. |
| |
| Keywords: | interleukin-12 Hela cell immune killing activity |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
