| 高效液相色谱法测定痛经康胶囊中延胡索乙素的含量 |
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| 引用本文： | 李广胜. 高效液相色谱法测定痛经康胶囊中延胡索乙素的含量[J]. 河南中医药学刊, 2010, 0(1): 108-109 |
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| 作者姓名： | 李广胜 |
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| 作者单位： | 河南中医学院第三附属医院,河南郑州450008 |
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| 摘 要： | 目的：建立以高效液相色谱法测定痛经康胶囊中延胡索乙素的含量方法。方法：色谱柱为AgilentHypersil ODS C18（250mm×4.6mm,5μm）,流动相为甲醇-0.04%磷酸溶液（50∶50）,流速为1.0mL·min^-1,检测波长为285nm,柱温30℃。结果：延胡索乙素检测浓度在0.0245-0.245mg·mL^-1范围内的线性关系良好（r=0.9999）;平均回收率为98.26%,RSD=1.10%（n=6）。结论：该方法简便、快速、准确,可用于痛经康胶囊的质量控制。
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| 关 键 词： | 高效液相色谱法 痛经康胶囊 延胡索乙素 含量测定 |
Determination of Tetrahydropalmatine Content in Tongjingkang Capsule by HPLC |
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| Li Guangsheng. Determination of Tetrahydropalmatine Content in Tongjingkang Capsule by HPLC[J]. Journal of Henan college of Traditional Chinese Medicine, 2010, 0(1): 108-109 |
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| Authors: | Li Guangsheng |
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| Affiliation: | Li Guangsheng (NO.3 Affiliated Hospital to the Henan College of Traditional Chinese Medicine,Zhengzhou,Henan,China 450008 ) |
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| Abstract: | Objective：To establish a HPLC method for the determination of tetrahydropalmatine in Tongjingkang Capsule.Methods：the determination was performed on Agilent Hypersil ODS C18 with the mobile phase consisted of methanol-0.04% H3PO4（50∶ 50） at a flow rate of 1.0 mL·min^-1.The detection wavelength was 285 nm and the colum temperature was 30℃.Results：The linear relation of tetrahydropalmatine was excellent within the range of 0.0245-0.245mg·mL^-1（r=0.9999）,and the average Recovery was 98.26% ,RSD=1.10%（n=6）.Conclusion：The method is simple,rapid,accurate,and Can be used for the quality control of Tongjingkangcapsule. |
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| Keywords: | HPLC Tongjingkang capsule tetrahydropalmatine content determination |
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