茎尖微嫁接技术脱除佛手病原体的研究

目的采用茎尖微嫁接技术脱除佛手病原体,为无病苗木的培育奠定基础。方法采用茎尖微嫁接技术获得了佛手微嫁接成活苗,应用PCR技术对其进行黄龙病病原进行检测,同时考察了茎尖大小对嫁接成活率和脱病原体率的影响。结果经PCR检测,微嫁接苗脱除了黄龙病病原;茎尖大小对嫁接成活率和脱病原体率的影响较大,带4个叶原基的茎尖嫁接成活率最高,达60.00%,脱病原体率为85.71%,带2个叶原基的茎尖,脱病原体率达100%,但嫁接成活率低。结论茎尖微嫁接技术可以脱除佛手病原体,综合考虑嫁接成活率和脱病原体的效果,宜选用带3～4个叶原基的茎尖进行脱病原体苗的生产。

Study on Pathogen-removal Shoot-tip Micrografting Technology of Citrus medica L.

Objective This paper is focused on how to lay a foundation of cultivation for virus-removed plantlets of C. medica L. by shoot-tip micrografting. Methods CMS plantlets were procured by shoot-tip micrografting with Huanglong disease detected by polymerase chain reaction （PCR） technology afterwards, and meanwhile an investigation was conducted on the effect of the sshoot-tip size on micrografting survival rate and pathogen-removal rate of CMS plantlets. Results Detected by PCR, it was showed that the pathogens of Huanglong disease had been removed from CMS plantlets after micrograft and that the size of the shoot- tip affected significantly on micrografting survival rate and pathogen-removal rate of CMS plantlets. The micrografting survival rate and pathogen-removal rate of 4-leaf primordia shoot-tips were respectively 60.00%（ the highest） and 85.71% while 2-leaf primordia ones were recorded a comparatively low micrografting survival rate and the highest pathogen-removal rate （100%）. Conclusion 3 or 4 leaf primordia shoot-tip micrografting can be applied in the mass production of pathogen-removed CMS plantlet, taken micrografting survival rate and pathogen-removal effect into consideration.