| 腊梅花中槲皮素的含量测定 |
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| 引用本文： | 吴斌. 腊梅花中槲皮素的含量测定[J]. 河南中医药学刊, 2010, 0(2): 262-263 |
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| 作者姓名： | 吴斌 |
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| 作者单位： | 上饶市立医院,江西上饶334000 |
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| 摘 要： | 目的：建立测定腊梅花中槲皮素含量的HPLC分析方法。方法：色谱柱：HypersilODSC18柱（250mm×4.6mm,5μm）;流动相：甲醇-水（65∶35）;流速1.0ml·min^-1,检测波长：λ=254nm;柱温为25℃;进样量为10μl。结果：槲皮素在0.2-2μg（R^2=0.9985）与峰面积线性关系良好。平均回收率为98.11%,RSD=2.08%。结论：HPLC分析方法操作简便、快速、准确,可用于腊梅花中槲皮素的含量测定。
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| 关 键 词： | 腊梅花 槲皮素 高效液相色谱 |
Determination of Quercetin in the Flowers of Chimonanthus Praecox by RP-HPLC |
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| Wu Bin. Determination of Quercetin in the Flowers of Chimonanthus Praecox by RP-HPLC[J]. Journal of Henan college of Traditional Chinese Medicine, 2010, 0(2): 262-263 |
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| Authors: | Wu Bin |
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| Affiliation: | Wu Bin( Medicine Hospital of Shangrao City,Shangrao,Jiangxi,China 334000) |
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| Abstract: | Objective：To establish a RP-HPLC method for the determination of quercetin in the flowers of Chimonanthus praecox.Methods：A RP-HPLC method was established and a Hypersil ODS C_18 column（250×4.6 mm,5 μm）was used.The mobile phase was methanol-water（65∶35）and detection wavelength was at 254 nm,The flow rate was 1.0 ml·min^-1 and the column temperature was at 25℃.Results：Quercetin had a good linearity in the range of 0.2-2 μg（R^2 = 0.9985）,and the recovery rate was 98.11% with RSD of 2.08%.Conclusion：This method is simple,accurate and repeatable.It can be used to determine quercetin in the flowers of Chimonanthus praecox. |
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| Keywords: | Chimonanthus praecox quercetin RP-HPLC |
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