舒胸片中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Rb_1的含量测定

目的建立舒胸片中三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法采用HPLC梯度洗脱法,使用C18柱,流动相为乙腈-水梯度洗脱,(0～5min,20%～25%乙腈;5～20min,25%～45%乙腈);ELSD漂移管温度70℃;氮气流速2.0L/min;柱温35℃。结果舒胸片中三七皂苷R1,人参皂苷Rg1和人参皂苷Rb1成分得到很好分离,线性关系良好,平均加样回收率:三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1分别为100.14%,99.77%和99.65%,RSD分别为1.07%,0.47%和1.06%。结论本法结果准确,便于操作,可作为舒胸片的质量控制方法之一。

Determination of the Main Components by HPLC-ELSD in Shuxiong Tablets

Objective To establish a method for determination of notoginsenodside R1, ginsenosideRg1 and ginsenosideRb1 in Shuxiong tablets. Methods HPLC-ELSD was used to determine on C18 column gradient eluted with acetonitrile and water as the mobile phase program, column temperature at 35 ℃. The elution program was （0-5min,20%-25% acetonitrile;5-20 min,25%-45% acetonitrile）;drift tube temperature was at 70 ℃, gas flow rate was 2.0L/min. Results 3 saponins were separated well. Average recovery was 100.14% for notoginsenodside RI, 99.77% for ginsenoside Rgl, 99.65% for ginsenoside Rb1, respectively. Conclusion The method is simple and rapid with satisfactory results. It is suitable for quality control of Shuxiong tablets.