新型有机硒化合物Eb诱导舌癌细胞凋亡的实验研究

目的　体外研究新型有机硒化合物Eb对人舌癌细胞Tca83的诱导凋亡作用。方法　体外培养人舌癌Tca83细胞,不同浓度的有机硒化合物Eb作用2 4h后,通过MTT (四唑氮盐)法和流式细胞仪检测细胞的存活率和凋亡率。结果　新型有机硒化合物Eb诱导细胞凋亡,凋亡率随浓度的增强而增加。各浓度组与对照组有显著性差异(P <0 . 0 5 ) ,各浓度组之间也有显著性差异(P <0. 0 5 )。结论　新型有机硒化合物Eb能够诱导细胞凋亡,并且有浓度依赖性。

The Study of A Novel Organoselenium Compounds (Eb) Inducing Apoptosis of Human Oral Squamous Cell Carcinoma Tca83 in Vitro

Objective To study A novel organoselenium compounds(Eb) induce apoptosis of human oral squamous cell carcinoma Tca83. Methods Human oral squamous cell carcinoma Tca83 were cultured in RPMI-1640 medium, which were dealed with the novel organoselenium compounds(Eb) with different doses. We calculate the survival rates of cell by MTT and apoptotic rates of cell by flow cytometer after 24h. Results The novel organoselenium compounds(Eb) induced apoptosis, and the apoptotic rates increased with the adding of doses, and every groups had obvious differences. Conclusions The novel organoselenium compounds(Eb) induced apoptosis, and the apoptotic rates depended on the doses.