Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro |
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Authors: | Yi Zeng Zhong Yang Jian-Guo Xu Meng-Su Yang Zhi-Xiong Zeng Chao You |
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Institution: | 1. Department of Diagnostics and Public Health, FISH Lab, AOUI Hospital Trust of Verona, Italy;2. Department of Diagnostics and Public Health, Anatomic Pathology, AOUI Hospital Trust of Verona, Italy;3. Anatomic Pathology, S. Chiara Hospital, Trento, Italy;4. Department of Surgical Sciences, Neurosurgery Unit, AOUI Hospital Trust of Verona, Italy;5. Oncology Unit, AOUI Hospital Trust of Verona, Italy;6. Anatomic Pathology, Cazzavillan Hospital, Arzignano, Vicenza, Italy;7. Oncology, Camposampiero—Cittadella, ULSS15 Padova, Italy;8. Anatomic Pathology, Pederzoli Hospital, Peschiera Del Garda, Verona, Italy |
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Abstract: | Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10 μM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression. |
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