| 母血HBV DNA含量与新生儿HBV感染关系 |
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| 引用本文： | 魏俊妮,王素萍,冯永亮,宋秀霞,李淑珍,李小燕,双杰玉.母血HBV DNA含量与新生儿HBV感染关系[J].中国公共卫生,2010,26(1). |
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| 作者姓名： | 魏俊妮 王素萍 冯永亮 宋秀霞 李淑珍 李小燕 双杰玉 |
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| 作者单位： | 1. 山西医科大学流行病学教研室,山西太原,030001
2. 山西省太原市传染病医院 |
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| 摘 要： | 目的 探讨HBsAg阳性孕妇外周血乙型肝炎病毒(HBV)DNA含量及细胞转运与新生儿HBV感染的关系.方法 用荧光定量PCR方法定量检测HBsAg阳性孕妇血清HBV DNA;用等位基因特异性PCR及半巢式PCR技术扩增HBsAg阳性孕妇新生儿外周血中母亲DNA,通过检测谷胱甘肽-S-转移酶(GSTM1)、血管紧张素转换酶(ACE)等位基因确定母-胎细胞转运;用巢式PCR检测新生儿血清、外周血单个核细胞(PBMC)中HBV DNA.结果 随着孕妇血清HBV DNA含量的增加,新生儿HBV DNA阳性的危险性呈现增高趋势(X~2=13.16,P<0.05,趋势X~2=12.42,P<0.05),而孕妇血清HBV DNA含量与新生儿PBMC HBV DNA阳性无关(总X~2=2.41,P>0.05,趋势X~2=0.35,P>0.05);孕妇血清HBV DNA含量与母-胎细胞转运无关(总X~2=4.14,P>0.05,趋势X~2=0.001,P>0.05);母-胎细胞转运与新生儿外周血PBMC HBV DNA阳性有关(X~2=10.26,P<0.05),而与新生儿外周血HBV DNA阳性无关(X~2=0.49,P>0.05).结论 HBsAg阳性孕妇血清HBV DNA含量、母-胎细胞转运与新生儿HBV感染有关,分别是新生儿血清、PBMC HBV感染的重要危险因素.
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| 关 键 词： | 乙型肝炎 等位基因 外周血单个核细胞 新生儿 |
Relationship between HBV DNA content in mother and HBV infection in newborn |
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| WEI Jun-ni,WANG Su-ping,FENG Yong-liang,et al..Relationship between HBV DNA content in mother and HBV infection in newborn[J].Chinese Journal of Public Health,2010,26(1). |
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| Authors: | WEI Jun-ni WANG Su-ping FENG Yong-liang |
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| Institution: | WEI Jun-ni,WANG Su-ping,FENG Yong-liang,et al.Department of Epidemiology,Shanxi Medical University(Taiyuan 030001,China) |
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| Abstract: | Objective To study the relationship between hepatitis B virus(HBV),DNA content in pregnant mother,mother-fetus cell transportion and HBV infection in newborn.Methods HBV DNA contents in blood of pregnant women were detected by real-time fluorescence quantitative PCR.DNA in mother and in fetus was amplified by allele-specific PCR and heminested PCR.The cell transportion of mother-fetus was determined by detecting GSTM1 and ACE.HBV DNA in newborns' peripheral blood serum and peripheral blood monouclear cells ... |
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| Keywords: | PCR HBV DNA hepatitis B allele peripheral blood mononuclear cell(PBMC) polymerase chain reaction(PCR) HBV DNA newborn |
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