肝脂消煎剂对脂肪肝大鼠肝脏脂质过氧化及膜流动性损伤的作用研究

目的：探讨肝脂消煎剂治疗脂肪肝药效学机制。方法：采用高脂饮食加白酒灌胃建立大鼠脂肪肝模型，以东宝肝泰作对照，观察肝脂消煎剂对脂肪肝大鼠肝微粒体、线粒体膜流动性及微粒体后上清SOD、MDA的影响。结果：模型对照组MDA、膜荧光偏振度较正常组明显增大，且二者呈正相关(r=O．9651、r=0．9746)，表示肝脏因脂质过氧化引起膜流动性降低；肝脂消组能提高肝脏SOD活牲，降低MDA含量及荧光偏振度，拮抗膜流动性下降，均明显优于东宝肝泰。结论：中药可抑制肝脏脂质过氧化，拮抗其膜流动性降低，具有良好的防治脂肪性肝炎作用。

Experimental Study on the Effect of Ganzhixiao Decoction on Hepatic Lipid Peroxidation and Membrane Fluidity Injury of Fatty Liver Rat

Objective:To investigate the pharmacodynamics mechanism of Ganzhixiao decoction in treating fatty liver.Methods:High fat diet and white wine perfusate were used to make the fatty liver rat model.The Dongbao Gantai was chosen to be the control medicine.The effect of Ganzhixiao decoction on hepatic microsome,mitochondrial membrane fluidity,superoxide dismutase(SOD) and (MDA) of fatty liver rat was observed.Results:Compared with normal control group,model control group appeared to be significantly enlarged in MDA and membrane fluorescence polarization degree,and it was correlate with the normal control group(r=0 9651,r=0 9746),which showed hepatic membrane fluidity was reduced by lipid peroxidation;Ganzhixiao decoction was better than Dongbao Gantai in reducing content of MDA and fluorescence polarization degree,antagonizing reduction of membrane fluidity,increasing activity of hepatic SOD.Conclusion:TCM may inhibit hepatic lipid peroxidation,antagonize reduction of membrane fluidity,and have better effect on preventing and treating fatty hepatitis.