金属蛋白酶组织抑制剂对涎腺腺样嚢性癌表达β-肌营养不良蛋白聚糖的影响

目的 研究涎腺腺样囊性癌(SACC)中β-肌营养不良蛋白聚糖(β-DG)的表达情况，探讨金属蛋白酶组织抑制 剂(TIMPs)对SACC细胞表达β-DG的影响。方法 采用免疫细胞化学染色法检测经不同浓度(10、15、20、25 μmol·L-1) TIMPs作用后SACC肺高转移株ACC-M和肺低转移株ACC-2中β-DG的表达情况，以未经TIMPs作用的ACC-M和ACC-2 细胞作为对照。收集7例SACC患者的肿瘤组织样本，以10例正常涎腺组织作为对照，采用免疫组织化学染色法检 测β-DG的表达情况。结果 未经TIMPs作用前，ACC-2和ACC-M细胞均未见β-DG表达，经不同浓度TIMPs作用后， ACC-2和ACC-M细胞β-DG表达均为阳性。10例正常涎腺组织中，β-DG主要表达于腺泡的基膜上；SACC组织的癌巢周围及癌细胞中β-DG表达阴性。结论 SACC细胞外基质和细胞骨架连接处的β-DG发生了断裂，可能使其更容易发生侵袭和转移，而TIMPs能够逆转β-DG的表达，为治疗SACC提供了新思路。

The effects of tissue inhibitor of metalloproteinases on p-dystroglycan in salivary adenoid cystic carcinoma

Objective To investigate the expression of β-dystroglycan (β-DG) and the roles of tissue inhibitor of metalloproteinases (TIMPs) on β-DG in salivary adenoid cystic carcinoma (SACC) . Methods β-DG in highly lung metastatic cell line ACC-M and lowly lung metastatic one ACC-2 was tested by immunocytochemistry with different concentrations (10, 15, 20, 25 μmol ? L-1) of TIMPs, and that without the regulation of TIMPs was served as controls. β-DG was detected in seven specimens of SACC and ten cases of normal salivary gland tissues which were considered as a comparison group by immunohistochemistry. Results There was no positive β-DG immune-staining at the ACC-2 and ACC-M cell lines without TIMPs in the cell culture. β-DG expressed after the regulation of TIMPs. β-DG expression was localized predominantly in basement membrane of the acinus, while the negative results were distributed in the carcinoma cells and around the cancer cell nests. Conclusion β-DG is widely expressed by transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton, the fracture of this structure means that it is easy to invade and transfer, so restoration of β-DG expression by TIMPs is considered to be critical for successful treatment of SACC.