Tiam 1与胃癌细胞侵袭转移及与裸鼠移植瘤形成的关系

摘要：目的 检测胃癌细胞中T淋巴瘤侵袭转移诱导因子 1(Tiam 1)的表达，并分析其与胃癌细胞离体、在体侵袭转移能力的关系。方法 采用层粘连蛋白黏附法，由胃癌MKN 45细胞株(M0)中筛选获得高(MH)、低(ML)黏附亚株。应用RT PCR和定量细胞ELISA技术分别检测Tiam 1 mRNA与蛋白在M0，ML，MH细胞中的表达；应用Boyden小室法测定M0，ML，MH细胞的离体侵袭移行能力，并分析其与Tiam 1表达的关系。应用裸鼠接种法观察M0，ML，MH细胞的在体成瘤及转移能力。结果 MH细胞中Tiam 1 mRNA(RV=0.855±0.051)与蛋白的表达(RD=1.262±0.165)以及其离体侵袭转移能力(24.33±8.02，52.00±14.53)、在体裸鼠肺转移率(4/5=80%)均较M0细胞(RV=0.759±0.047，RD=0.911±0.104，11.67±3.79，26.00±9.54，2/5=40%)，ML细胞(RV=0.743±0.039，RD=0.892±0.101，9.67±3.06，23.67±8.50，1/5=20%)为强，统计学差异显著(P< 0.05)，但在M0，ML细胞间无统计学差异(P> 0.05)；Tiam 1表达水平与胃癌细胞的侵袭转移能力呈完全及高度正相关(P< 0.05)。结论 Tiam 1表达水平升高有可能促进胃癌细胞侵袭转移能力的增强。

Correlation of Tiam 1 to invasion and metastasis of gastric cancer cells and to development of tumor xenografts in nude mice

Abstract:Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1(Tiam 1) in gastric cancer cells and analyse the correlation between Tiam 1 and the invasive and migratory potential of gastric cancer cells in vitro and in vivo.Methods Two subpopulations lesser(ML) and higher(MH) adhesive subgroup were separated from human gastric cancer cell line MKN 45(M0) by laminin adhesion method in vitro. RT PCR and ELISA were applied to detect the expression of Tiam 1 mRNA and protein in M0, ML and MH cells. The invasive and migratory potential of M0, ML and MH cells in vitro and in vivo were observed by Boyden chamber and by inoculation into nude mice and, simultaneously, correlation between the expression of Tiam 1 and the invasive and migratory potential of gastric cancer cells was analysed.Results The invasive and migratory potential of MH cells in vitro (24.33±8.02, 52.00±14.53) and the lung metastatic rate of MH cells in nude mice (4/5 = 80%) was much higher than that of M0 cells (11.67±3.79, 26.00±9.54, 2/5 = 40%, RV = 0.759±0.047, RD = 0.911±0.104) and ML cells (9.67±3.06, 23.67±8.50, 1/5 = 20%, RV = 0.743±0.039, RD = 0.892±0.101) (P< 0.05), 〖JP3〗as well as the expression of Tiam 1 mRNA (RV = 0.855±0.051) and protein (RD = 1.262±0.165) in MH cells were much higher than those in MO and ML cells (P< 0.05),