| 氨基胍对全肝血流阻断再灌注大鼠肺损伤的影响 |
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| 引用本文： | 曹德权,陈艳平,常业恬,李永国. 氨基胍对全肝血流阻断再灌注大鼠肺损伤的影响[J]. 中南大学学报(医学版), 2006, 31(1): 94-96 |
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| 作者姓名： | 曹德权 陈艳平 常业恬 李永国 |
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| 作者单位： | 中南大学湘雅二医院,麻醉科,长沙,410011 |
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| 摘 要： | 目的：探讨氨基胍（AG）对全肝血流阻断再灌注大鼠肺损伤的影响。方法：阻断肝门及肝上、肝下下腔静脉20min建立大鼠全肝血流阻断再灌注模型。将90只大鼠随机均分成假手术组（A组）、全肝血流阻断组（B组）和全肝血流阻断加氨基胍治疗组（C组），每组再根据观察时间段的不同随机均分成3个亚组。A，B两组按1mL／kg从股静脉注射生理盐水，C组注射AG20mg／kg（溶于1mL／kg生理盐水中）。观察各组全肝血流阻断20min（T0）、再灌注4h（T1）门静脉血一氧化氮（NO）、内毒素（ET）、肿瘤坏死因子（TNF-α）浓度，大鼠48h生存率，大鼠肺组织湿千质量比及电镜下组织结构变化。结果：与A组比较，B，C两组T0．T1门静脉血NO，ET，TNF-α及肺湿干质量比明显升高（P〈0．05或P〈0，01），但C组明显低于B组（P〈0．05）；B，C两组大鼠48h存活率降低，但C组明显高于B组（P〈0．05）；B，C两组肺组织均存在病理改变，但C组明显轻于B组。结论：氨基胍具有保护全肝血流阻断再灌注大鼠肺组织的作用。
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| 关 键 词： | 氨基胍 肝脏 缺血再灌注 肺 大鼠 |
| 文章编号： | 1672-7347（2006）01-0094-03 |
| 收稿时间： | 2005-04-08 |
| 修稿时间： | 2005-04-08 |
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| CAO De-quan,CHEN Yan-ping,CHANG Ye-tian,LI Yong-guo.
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| Authors: | CAO De-quan CHEN Yan-ping CHANG Ye-tian LI Yong-guo |
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| Affiliation: | Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. caodequan989@hotmail.com |
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| Abstract: | OBJECTIVE: To investigate the effects of aminoguanidine on the lung injury induced by the total hepatic ischemia-reperfusion in rats. METHODS: The total hepatic ischemia-reperfusion model was built after blocking of the hepatic porta, suprahepatic and infrahepatic vena cava. Ninety Sprague-Dawley rats were assigned randomly into 3 groups: Sham operation group (Group A, n=30); total hepatic ischemia group (Group B, n=30); and aminoguanidine treatment group (Group C, n=30). Each group was subdivided randomly into 3 subgroups (n=10) according to different time phases: 20 minutes after the total hepatic vascular exclusion (T0), 4 hours after the reperfusion (T1), and 48 hours after the survival Group A and Group B were intravenously injected with normal saline ( mL/kg) while Group C was injected with aminoguanidine (20 mg/kg) dissolved in normal saline (1 mL/kg) 10 minutes before the open of the abdomin. The levels of portal blood nitric oxide ( O) endotoxin ( ET), tumor necrosis factor-alpha (TNF-alpha at T0 and T1 were detected; 48 hours survival rates and the lung wet/dry weight ratio were counted; and the histological changes of the lung tissues were observed. RESULTS: Compared with Group A, the levels of portal vein NO, ET, and TNF-alpha T0 and T1 in Group B and Group C were significantly higher (P < 0.05 or P < 0.01). But those indexes of Group C were lower than those of Group B (P < 0.05). The 48-hour survival rate in Group C was higher than that in Group B (P < 0.05). The lung wet/dry weight ratio in Group C was lower than in Group B (P < 0.05) and the histological change of Group C was slighter than that in Group B. CCONCLUSION: Aminoguanidine has the protective effects on the lungs against the total hepatic ischemia-reperfusion induced injury. |
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| Keywords: | aminoguanidine liver ischemia-reperfusion lung rats |
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