大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选

目的:优选大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺条件.方法:采用HPLC测定紫丁香苷含量,通过静态吸附-洗脱试验考察大孔树脂型号,利用单因素试验考察上样量、吸附流速、洗脱机浓度及用量对纯化工艺的影响.结果:选用XDA-1型大孔树脂,其纯化工艺条件为上样量每g树脂吸附3.5 mg紫丁香苷,吸附流速1 BV·h-1,用9 BV 40％乙醇于2BV·h-1流速洗脱,紫丁香苷纯度由5.4％提高到23.1％.结论:XDA-1型大孔树脂可用于纯化倒卵叶五加提取液,紫丁香苷收率和纯度均达满意效果.

Optimization of Separation and Purification Technology of Syringin in Acanthopanax obouatus with Macroporous Absorption Resin

Objective: To optimize separation and purification technology of syringin in Acanthopanax obouatus with macroporous absorption resin. Method: With the content of syringin as index,which was determined by HPLC,model of macroporous absorption resin was screened by static adsorption-elution test,purification conditions were investigated by single factor tests. Result: XDA-1 macroporous adsorption resin model was adopted,its purification technology was as following:the amount of sample 3.5 mg syringin with 1 g XDA-1 macroporous absorption resin,adsorption velocity 1 BV·h^-1,eluted with 9 BV 40% ethanol at 2 BV·h^-1.Purity of syringin was up to 28.14% from 5.47%. Conclusion: XDA-1 macroporous resin could be used to purify extract of A. obouatus,yield and purity of syringin obtained satisfactory results.