二年生黄芩生长发育及有效成分动态研究

目的:研究承德地区二年生黄芩生长发育及有效成分动态积累变化规律.方法:2008年5月1日到10月15日每隔15 d取样一次,并记录物候期,所取黄芩样品采用高效液相色谱法测定其黄芩苷、黄芩素和汉黄芩素含量,用紫外分光光度法测定其总黄酮含量.结果:承德地区二年生黄芩地上部有两次营养生长,总的生长规律是慢-快-慢.根部总的生长规律是快-慢-快,折干率在枯萎期最高达到55.31％.黄芩苷和总黄酮含量的变化相似,即5月中旬最高,分别为17.61％,42.92％,黄芩素和汉黄芩素含量相似,即6月份最高,分别是2.63％,0.49％.结论:承德二年生黄芩黄芩苷和总黄酮5月中旬最高,黄芩素和汉黄芩素6月份最高,折干率10月中旬最高.

A Study on Dynamics of Growth and Development and Active Component of Biennial Scutellaria Baicalensis Georgi

Objective: To study the change rule of dynamic accumulation of biennial Scutellaria baicalensis growth and development and active component in Chengde regions. Method: Took samples once every fifteen days from 1 May to 15 October 2008 and recorded the phenophase. Using high performance liquid chromatographic method to measure the content of baicalin, baicalein and wogonin, and using ultraviolet spectrophotometry to measure its flavonoids content. Result: The general growth rule of aerial parts of biennial S. baicalensis in Chengde regions was slow-fast-slow. Which was in vegetative growth two times. The general growth rule of root was fast-slow-fast, which had highest drying rate on 55.31%. The changes of content of baicalin and flavonoids were similar, which the highest content was at middle May, and the data were 17.61%and 42.92%. The changes of content of baicalein and wogonin were similar, which the highest content was at at June, and the data were 2.63% and 0.49%. Conclusion: The highest content of baicalin and flavonoids in biennial S. baicalensis which was grown in Chengde regions was at middle May. The highest content of wogonin and flavonoids was at June. The highest drying rate was in early and middle October.