| 鳖甲水提物W-A-80在模拟胃肠液中消化情况分析 |
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| 引用本文： | 韩秋俊,徐士勋,王鹏龙,王艳慧,程亚涛,周燊,褚福浩,宋霁翔,李国梁,李强,雷海民.鳖甲水提物W-A-80在模拟胃肠液中消化情况分析[J].中国实验方剂学杂志,2013,19(14):23-27. |
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| 作者姓名： | 韩秋俊 徐士勋 王鹏龙 王艳慧 程亚涛 周燊 褚福浩 宋霁翔 李国梁 李强 雷海民 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金项目(81073017);北京中医药大学科研创新团队(2011-CXTC-15) |
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| 摘 要： | 目的:研究鳖甲水提物W-A-80在模拟胃、肠环境中消化情况,探讨其体内转化形式.方法:采用水提醇沉法制备鳖甲水提物W-A-80,模拟胃肠液环境,于37℃消化.以寡肽产率为指标,通过双缩脲反应-酶联免疫检测仪法考察胃蛋白酶和胰蛋白酶于不同酶底比、酶解时间的寡肽(截留相对分子质量＜3 500)产率变化；平衡透析法测定模拟胃肠液消化后所得寡肽与体外小鼠肝组织的蛋白结合率.结果:确定酶解条件为人工胃液酶底比1∶10,于37℃酶解3h,不同质量浓度寡肽与肝组织的蛋白结合率分别为31.63％,19.93％,9.65％；人工肠液酶底比1∶100,于37℃酶解11h,不同质量浓度寡肽与肝组织的蛋白结合率分别为48.75％,47.43％,22.72％;均明显高于空白对照的7.00％,5.31％,4.60％.结论:鳖甲水提物W-A-80经模拟胃肠液消化有大量寡肽产生,且活性显著强于原液,推测寡肽可能是鳖甲抗肝纤维化的物质基础.
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| 关 键 词： | 鳖甲 模拟胃液 模拟肠液 寡肽 肝组织蛋白结合率 |
| 收稿时间： | 2013/1/27 0:00:00 |
Digestion Analysis of Water Extract W-A-80 from Trionycis Carapax in Simulated Gastrointestinal Fluid |
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| HAN Qiu-jun,XU Shi-xun,WANG Peng-long,WANG Yan-hui,CHENG Ya-tao,ZHOU Shen,CHU Fu-hao,SONG Ji-xiang,LI Guo-liang,LI Qiang and LEI Hai-min.Digestion Analysis of Water Extract W-A-80 from Trionycis Carapax in Simulated Gastrointestinal Fluid[J].China Journal of Experimental Traditional Medical Formulae,2013,19(14):23-27. |
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| Authors: | HAN Qiu-jun XU Shi-xun WANG Peng-long WANG Yan-hui CHENG Ya-tao ZHOU Shen CHU Fu-hao SONG Ji-xiang LI Guo-liang LI Qiang and LEI Hai-min |
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| Institution: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | |
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| Keywords: | Trionycis Carapax simulated gastric fluid simulated intestinal fluid oligopeptides liver tissue protein binding rate |
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