| 白屈菜红碱、血根碱单体的分离和鉴定 |
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| 引用本文： | 冯泓瑞,黄松,陈玲玲,鲁芹飞,林吉,赖小平.白屈菜红碱、血根碱单体的分离和鉴定[J].中国实验方剂学杂志,2013,19(14):36-38. |
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| 作者姓名： | 冯泓瑞 黄松 陈玲玲 鲁芹飞 林吉 赖小平 |
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| 作者单位： | 广州中医药大学新药研发中心,广州,510006 |
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| 基金项目： | 国家科技支撑计划项目(2012BAI29B00) |
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| 摘 要： | 目的:分离及鉴定白屈菜红碱和血根碱单体.方法:采用柱色谱分离技术对白屈菜红碱和血根碱进行分离纯化,通过HPLC进行鉴定.结果:采用柱色谱法可获得单一成分,经HPLC鉴定为白屈菜红碱和血根碱单体,纯度分别为98.6％,95.7％.结论:采用柱色谱技术可从博落回中提取、分离到高纯度的白屈菜红碱和血根碱单体.
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| 关 键 词： | 柱色谱 白屈菜红碱 血根碱 博落回 高效液相色谱法 |
| 收稿时间： | 2013/1/17 0:00:00 |
Separation and Identification of Chelerythrine and Sanguinarine Monomer |
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| FENG Hong-rui,HUANG Song,CHEN Ling-ling,LU Qin-fei,LIN Ji and LAI Xiao-ping.Separation and Identification of Chelerythrine and Sanguinarine Monomer[J].China Journal of Experimental Traditional Medical Formulae,2013,19(14):36-38. |
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| Authors: | FENG Hong-rui HUANG Song CHEN Ling-ling LU Qin-fei LIN Ji and LAI Xiao-ping |
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| Institution: | New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;New Drug Research and Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | Objective: To isolate and identify chelerythrine and sanguinarine monomer. Method: Chelerythrine and sanguinarine were isolated and purified by column chromatography technology,and identified by HPLC. Result: Single component could be obtained by column chromatography method,chelerythrine and the sanguinarine monomer were identified by HPLC with purity of 98.6%,95.7%,respectively. Conclusion: Column chromatography technology could obtain high purity chelerythrine and sanguinarine monomer from Macleaya cordata. |
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| Keywords: | column chromatography chelerythrine sanguinarine Macleaya cordata HPLC |
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