肝细胞癌耐药表达与转移潜能的关系

目的 研究肝细胞癌（ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ，ＨＣＣ）多药耐药（ｍｕｌｔｉｄｒｕｇ ｒｅｓｉｓｔａｎｃｅ，ＭＤＲ）基因表达蛋白Ｐ－糖蛋白（Ｐ－ｇｌｙｃｏｐｒｏｔｅｉｎ，Ｐ－ｇｐ）与转移能力的关系。方法 用链霉亲合素一生物素酶复合物（ｓｔｒｅｐｔ ａｖｉｄｉｎ－ｂｉｏｔｉｎ ｃｏｍｐｌｅｘ，ＳＡＢＣ）免疫组化方法，检测５７例ＨＣＣ标本中ｐ－ｇｐ，ｎｍ２３／ＮＤＰＫ，ｒａｓ／ｐ

Relationship between P- glycoprotein and metastatic potential in hepatocellular carcinoma

Objective: Whether the phenotypes of drug resistance and metastatic activity in cancer are dependent on each other or not is controversial. We studied the relationship between P-glycoprotein and metastatie activity in hepatocellular carcinoma (HCC). Methods: Fifty-seven histologically histologically verified HCC specimens were of obtained. SABC immunohistochemical staining with monoclonal antibody against human P-gp,, E-cad, Laminin, and with multiclonal antiboly against human NDPK, ras /p21. respectively, were usd to observe the P-gp, E-cad,Laminin, NDPK, p21 in all specimens. Results: nm23 /NDPK, ras/p21, Laminin and E-cad positive rate in P-gp(+) specimens were 43. 3%. 76.7%. 23.3% and 60%. while in P-gp(-), were 77.7%. 44.4%. 33.3% and 44.4%, respectively(x~2 value was 7.1. 6. 23, 0.7 and 1.38, P, value was <0.01. < 0.05, > 0.05 and > 0.05, respectively).The incidence of invasiveness and metastasis in P-gp(+) was 70. 0%. in p-gp (-) was 40. 7% (X2=4.94, p < 0.05 ). Conclusion:Our data suggest that overexpression of P-gp in HCC may covariate with the phenotypes of both enhanced metastasis and low nm23 expression and high ras expression.