牻牛儿苗种质资源遗传多样性的ISSR研究

目的:研究牻牛儿苗亲缘关系和遗传多样性.方法:23个样品进行简单序列重复区间(ISSR)分析,利用NTSYS软件计算材料间遗传相似系数,并用UPGMA方法聚类,构建亲缘关系系统图.结果:从100条ISSR引物中筛选出14条多态性明显、反应稳定的引物,在23份供试材料DNA中共扩增出185条谱带,其中多态性条带142条,占76.8％.材料间ISSR标记遗传相似性系数(GS)在0.458 ～0.916.聚类分析结果显示,所有供试材料均可区分开,并聚为6组.结论:分子标记研究结果与牻牛儿苗的地理位置、形态性状存在显著的相关性,较好地揭示了牻牛儿苗种源间的遗传关系,可为牻牛儿苗资源划分和良种选育提供科学依据.

Genetic Diversity of Erodium stephanianum Based on ISSR Analysis

Objective: This article assessed the genetic relationship and genetic diversity in Erodium stephanianum. Method: Twenty-three germplasmic resources of E. Stephanianum. were analyzed by inter simple sequence repeat(ISSR) molecular markers. Genetic similarities were calculated by using NTSYS software and the dendrogram was constructed by using UPGMA method. Result: Fourteen ISSR primers were selected from 100 ISSR primers,and 185 DNA fragments were amplified from 23 samples.Of which,142 fragment were polymorphic(percentage of polymorphic bands was 76.8%). The genetic similarity among all accessions ranged from 0.458 to 0.916.Clustering analysis showed that the 23 samples of Fritillaria could be distinctively classified into 6 groups. Conclusion: Results from the cluster analysis were correlated significantly with the morphological characteristics and geographical location of 23 samples. The data indicate that ISSR technique is useful to determine genetic diversity and genetic relationship among E. stephanianum. provenances, providing a scientific basis for genetic breeding,differentiation and new cultivar selection.