苦参碱对宫颈癌Hela细胞增殖、凋亡及Survivin基因表达的影响

目的:探讨苦参碱对宫颈癌Hela细胞增殖、凋亡的影响及其可能的分子机制.方法:应用不同浓度的苦参碱作用于人宫颈癌Hela细胞,采用MTT法检测细胞增殖；Annexin V FITC/PI双染色流式细胞仪检测细胞凋亡和细胞周期；RT-PCR和Western blotting分析Survivin基因表达.结果:苦参碱对Hela细胞的体外增殖具有抑制作用,量效关系显著,与对照组比较有统计学差异(P＜0.01),半数抑制浓度(IC50)为2.60 g·L-1.经流式细胞仪检测表明,苦参碱能使Hela细胞G0-G1期逐渐增加,G2-M期和S期逐渐减少,并且随着剂量的增加,Hela细胞凋亡率明显增加(P＜0.01).苦参碱对Hela细胞Survivin 基因表达有一定的抑制作用(P＜0.01),并呈剂量依赖性.结论:苦参碱能有效抑制人宫颈癌Hela细胞的增殖,诱导其凋亡,其作用机制可能与抑制Survivin基因的表达有关.

Effects of Matrine on Proliferation, Apoptosis and Survivin Gene Expression in Human Cervical Cancer Hela Cells

Objective: To investigate the effects of matrine on proliferation, apoptosis in human cervical cancer Hela cells and its possible molecular mechanism. Method: Hela cells were treated with matrine of different concentrations. MTT assay was used to measure the cell proliferative effect. The Annexin V FITC/PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle. RT-PCR and western blotting were performed to detect the expression of Survivin gene. Result: Matrine could inhibit the proliferation of Hela cells in vitro, and dose-effect relationship was significant (P<0.01). IC₅₀ of Hela cells was 2.60 g·L^-1. The flow cytometry test indicated that matrine could make Hela cells G₀-G₁ phase gradually increasing, G₂-M period and S phase reduce gradually, and with the increase of the dose, Hela cells apoptosis rate increased significantly (P<0.01). Matrine could suppress the expression of Survivin gene (P <0.01), and with the dose-dependent manner. Conclusion: Matrine could effectively inhibit proliferation and induce apoptosis of Hela cells. Its molecular mechanisms might be related to modulating the expression of Survivin.