槲皮素-7-硫酸酯钠对H2O2所致DNA氧化损伤的保护作用

目的:探讨槲皮素-7-硫酸酯钠的体外抗氧化活性及对DNA氧化损伤的保护作用.方法:通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基(·DPPH)、羟自由基(OH·)和超氧阴离子自由基(O2-·)清除实验评价槲皮素-7-硫酸酯钠的体外抗氧化活性；采用紫外扫描法、凝胶电泳技术观察槲皮素-7-硫酸酯钠对H2O2所致DNA氧化损伤的保护作用.结果:自由基清除实验结果显示,槲皮素-7-硫酸酯钠能有效清除·DPPH、·OH和O2-·自由基,其清除能力较维生素C(VC)及槲皮素都强,且呈浓度依赖性.DNA氧化损伤保护实验结果显示,H2O2可致小牛胸腺DNA出现断裂梯带和浓度下降,槲皮素-7-硫酸酯钠预处理可抑制H2O2所致的小牛胸腺DNA断裂及浓度下降,且有浓度依赖性.结论:槲皮素-7-硫酸酯钠体外有较强的抗氧化能力,并对H2O2所致的DNA氧化损伤有保护作用.

Protective Effect of Sodium Quercetin-7-mono-sulfonate on H2O2 Induced DNA Oxidative Damage

Objective: To provide a theoretical basis for clinical application of sodium quercetin-7-mono-sulfonate (SQMS), its antioxidant property and DNA oxidative damage protective effect in vitro were studied. Method: The antioxidant activity of SQMS was evaluated by 1,1-diphenyl-2-picryl-hydrazyl (·DPPH), hydroxyl radical (OH·) and superoxide radical (·O₂^-) scavenging assays. Gel electrophoresis and spectrophotometry methods were used to study the protective effect of SQMS on H₂O₂ induced DNA oxidative damage in vitro. Result: SQMS exhibited a concentration-dependent scavenging effect on·DPPH,·OH and O₂^-·, and its scavenging effects are stronger than vitamin C(VC) and quercetin. By gel electrophoresis and spectrophotometry, the DNA ladder disappeared and the DNA concentration obvious increased by treatment with H₂O₂.Pretreatment with quercetin or sodium quercetin-7-sulfonate, the DNA ladder was more clear and the concentration of DNA decreased obviously. Conclusion: SQMS has stronger antioxidant activity in vitro, and has protective effect on H₂O₂induced DNA oxidative damage.