中风防治灵丸对脑缺血大鼠神经功能及脑水肿的影响

目的:观察中风防治灵丸对脑缺血大鼠神经功能、脑含水量、脑梗死范围及脑组织病理形态学的影响.方法:60只键康雄性SD大鼠随机分为假手术组、缺血模型组、中风防治灵丸高、中、低剂量组(5.184,2.592,1.296 g·kg-1)、步长脑心通对照组(0.691 g·kg-1),每组10只,从术前7d开始灌胃,第8天造模,至处死前每天给药,手术前后疗程11d,分别于造模术后6,24,72 h对大鼠神经功能缺损情况评分；于术后72 h进行氯化三苯四氮唑(TTC)染色测量脑梗死范围,干湿重法测量脑含水量,常规HE染色观察脑组织病理形态变化.结果:与模型组比较,中风防治灵丸高、中剂量组动物的神经功能缺损程度显著改善,并能显著降低大鼠局灶性脑缺血后的脑梗死范围,均有显著性差异(P ＜0.05,P＜0.01)；中风防治灵丸高、中、低剂量均可显著降低大鼠局灶性脑缺血后的脑含水量,减轻脑水肿,与模型组相比均有显著性差异(P＜0.05,P＜0.01)；中风防治灵丸高、中剂量能明显改善脑缺血造成的大鼠脑组织损伤,减轻神经细胞的继发性损害.结论:中风防治灵丸对大鼠局灶性脑缺血损伤具有良好的保护作用.

The Effect of Zhongfeng Fangzhiling Pill on Neurological Function and Cerebral Edema of in Rat with Cerebral Ischemia

Objective: To observe the effect of Zhongfeng Fangzhiling Pill (ZFP) on neurological function, brain water content, cerebral infarction and pathological morphology in rat with cerebral ischemia. Method: Sixty rats were randomly divided in sham group, model group, high dose group of ZFP (5.184 g·kg^-1), middle dose group of ZFP (2.592 g·kg^-1), low dose group of ZFP (1.296 g·kg^-1) and Naoxintong (NXT) group (0.691 g·kg^-1). There were 10 rats in each group.Rats were taken medicine by gastric gavage in 7 days before operation. The cerebral ischemia model was established by blocking middle cerebral artery in rat with suture method at 8th day. The course of treatment was 11 days before and after operation.Score of nervous symptoms in rats was evaluated at 6, 24 and 72 h after operation by improved Zealonga 5 class. The cerebral infarction area was measured by 2,3,5 -triphenyltetrazoliumcheoride(TTC) staining at 72 h after operation. The brain water content was calculated through weighing anhydrous brain and hydrous brain at 72 h. The pathological change of brain tissue was analyzed by HE staining at 72 h. Result: Compared with model group, score of nervous symptoms was obviously improved and cerebral infarction area was reduced significantly in high dose and middle dose groups of ZFP (P<0.05,P<0.01). Brain water content was decreased obviously in all groups of ZFP (P<0.05,P<0.01). The degree of cerebral tissue injury in all groups of ZFP was less than that of in model group by observing brain tissue slice. Conclusion: ZFP could protect cerebral tissue in rat with localized cerebral ischemia.