HPLC测定消炎止咳片中吗啡的含量

目的:建立消炎止咳片中吗啡的HPLC测定方法.方法:采用高效液相色谱法,Sinochrom-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0..1 mol·L-1磷酸二氢钾溶液-甲酸(7.5∶92.5∶0.1),流速0.7 mL· min-1,检测波长284 nm.结果:吗啡在42.40 ～ 424.0 mg· L-1线性关系良好(r =0.999 9),平均加样回收率为100.25％,RSD 1.67％.结论:采用HPLC测定吗啡,方法简单、灵敏、准确,可用于消炎止咳片中吗啡的含量测定.

Determination of Morphine in Xiaoyanzhike Tablets by HPLC

Objective: To establish a method of high performance liquid chromatography (HPLC) for the determination of morphine in Xiaoyanzhike Tablets. Method: The method of high performance liquid chromatography was carried out on a column of Sinochrom-C₁₈ using the mobile phase of acetonitrile-0.1 mol·L^-1 potassium dihydrogen phosphate solution-methanoic acid (7.5:92.5:0.1) with a flow rate of 0.7 mL·min^-1. The detection wavelength was set at 284 nm. Result: The calibration curve of morphine was linear over the range of 42.40-424.0 mg·L^-1(r=0.999 9). The average recovery was 100.25% (RSD 1.67%). Conclusion: The method is simple and feasible, which can be used to determine the content of morphine in Xiaoyanzhike Tablets.