艾片口服给药后在小鼠体内的组织分布

目的: 建立龙脑在小鼠组织中GC-FID含量测定方法,考察艾片口服给药后在小鼠体内的组织分布情况。 方法: 小鼠按30.0 mg·kg^-1灌胃给予艾片,分别于给药后1,3,5,10,20,30,60,90,120 min脱颈椎处死小鼠,迅速分离出小鼠全脑、心、肝、脾、肺、肾,匀浆后以十八烷为内标,乙酸乙酯萃取,采用GC测定各组织中龙脑质量浓度。 结果: 建立的含量测定方法中萃取回收率、日内和日间精密度、稳定性均符合生物样品的分析要求。艾片口服给药后分布于各主要脏器,组织中龙脑含量顺序为肝>肾>心>脑>脾>肺,龙脑在肝、肺、脾中达峰时间明显早于心、脑、肾。 结论: 建立的GC-FID适用于组织中龙脑的含量测定,艾片在不同组织内分布存在差异,在肝、肾、心、脑等血流丰富的组织中含量较高。

Tissue Distribution of Borneol in Mice after Oral Administration

Objective: To develop a GC-FID method for determination of borneol concentration in mice tissues and investigate its tissues distribution after oral administration. Method: Wholebrain,heart,liver,spleen,lungs and kidneys were quickly isolated by executing mice at 1,3,5,10,20,30,60,90,120 min after oral administration of borneol with a dosage of 30.0 mg·kg^-1.These tissues were extracted with ethyl acetate and detected by GC-FID,octadecane was used as an internal standard. Result: Calibration curve was good linear,extraction recoveries,RSD of within-day and between-day with low,medium and high concentration were satisfied for analysis requirements of biological samples.Borneol distributed in all major organs after administration with the order of liver>kidney>heart>brain>spleen>lung,Tmax in liver,lung and spleen was earlier than in heart,brain and kidney. Conclusion: Established GC-FID could be applied to determination of borneol in tissues.There were differences of borneol distribution in each tissues,it mainly distributed in abundant blood-supply tissues such as liver,kidneys,heart and brain.