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| 芫花酯甲的快速TLC纯化技术与鉴定 | |
| 引用本文： | 王玲,李娆娆,孙婷婷,刘延泽. 芫花酯甲的快速TLC纯化技术与鉴定[J]. 中国实验方剂学杂志, 2013, 19(15): 41-43 |
| 作者姓名： | 王玲 李娆娆 孙婷婷 刘延泽 |
| 作者单位： | 1. 河南中医学院药学院,郑州,450008 2. 中国中医科学院中药研究所,北京,100700 3. 中国医学科学院药用植物研究所,北京,100193 |
| 基金项目： | 北京自然科学基金项目(7112097) |
| 摘 要： | 目的:制备高纯度、低成本的芫花酯甲对照品.方法:采用硅胶柱-制备TLC制备芫花酯甲单体,通过HPLC分析纯度,NMR确定结构.结果:硅胶柱-制备TLC比制备液相成本低,未发现可见杂质斑点,所得单体纯度较好.结论:该方法解决了本品在分离纯化方面高消耗低产出的难题,为芫花萜类化合物的研究与开发提供参考. |
| 关 键 词： | 芫花酯甲 制备TLC 纯化技术 |
| 收稿时间： | 2012-11-28 |
Rapid TLC Purification and Identification of Yuanhuacine |
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| WANG Ling,LI Rao-rao,SUN Ting-ting and LIU Yan-ze. Rapid TLC Purification and Identification of Yuanhuacine[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(15): 41-43 | |
| Authors: | WANG Ling LI Rao-rao SUN Ting-ting LIU Yan-ze |
| Affiliation: | College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China |
| Abstract: | Objective: To prepare yuanhuacine reference with high purity and low cost. Method: Yuanhuacine was obtained by Silica gel column-preparative TLC method.Purity of yuanhuacine was determined by RP-HPLC and structure was identified by NMR. Result: Cost of silica gel column-preparative TLC method was lower than preparative HPLC,no visible impurity spot,purity of prepared yuanhuacine was good. Conclusion: Problem of high cost,low yield of yuanhuacine in preparation could be resolved by this method,it provided a reference for research and development of terpenoids in Daphne genkwa. |
| Keywords: | yuanhuacine preparative TLC purification technology |
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