附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响

目的:观察附子理中汤对腹泻型肠易激(D-IBS)大鼠模型结肠组织中toll样受体4(TLR4)表达水平的影响.方法:健康SPF级大鼠50只随机分为正常组、模型组、匹维溴铵组、附子理中汤高、低剂量组,用束缚应激加大黄ig方法联合复制D-IBS大鼠模型,造模成功后附子理中汤高、低剂量组(30,15 g·kg-1)、匹维溴铵(30 mg·kg-1)ig给药,1次/d,连续14 d.采用荧光定量PCR方法观察该模型大鼠结肠黏膜TLR4 mRNA的表达情况.结果:模型组大鼠肠黏膜的TLR4的表达(1.229±0.172)高于正常对照组(1.109±0.062) (P ＜0.01).而附子理中汤的干预可使大鼠肠黏膜的TLR4的表达趋于正常,与正常组无明显差异.附子理中汤高(1.068±0.056),低(1.067±0.071)剂量组之间没有显著差异,匹维溴铵组(1.205±0.290)与正常组相比TLR4的表达增多(P＜0.01).结论:附子理中汤可能通过增强整体机体耐受性,降低TLR4的表达,减少免疫应答,来维持肠道的稳态.

Expression of TLR4 mRNA in Colon Tissue of Rat Diarrhea Type Irritable Bowel Model by Fuzi Lizhong Decoction

Objective: To observe the toll like receptor(TLR)4 mRNA expression decoction on rat diarrhea type irritable bowel syndrome (D-IBS)colon tissue model by Fuzi Lizhong(FZLZ) decoction. Method: SPF 50 rats were randomly divided into normal group, model group, FZLZ high and low dose group(30,15 g·kg^-1), pinaverium bromide group(30 mg·kg^-1). IBS rat model was made by restraint stress combined with ig rhubarb. After modeling,corresponding drugs were given at the dose, once a day for 14 days. TLR4 mRNA expression was observed through PCR method. Result: The expression of TLR4 in the model group (1.229±0.172)was higher than that in the control group(1.109±0.062)(P<0.01). While the expression of TLR4 in the FZLZ decoction was similar to normal groups, there was no significant difference between the high (1.068±0.056) and low (1.067±0.071)dose group, pinaverium bromide group(1.205±0.290) compared with the normal group TLR4 expression increased (P<0.01). Conclusion: FZLZ decoction may maintain intestinal homeostasis by enhancing the whole body tolerance, and decreaseing the expression of TLR4 and immune response.