不同产地藏药余甘子总鞣质含量测定

目的:建立磷钼钨酸/干酪素分光光度法测定不同产地藏药余甘子中总鞣质含量的方法.方法:以没食子酸为对照品,磷钼钨酸为显色剂,检测波长760 nm,测定8个不同产地余甘子中总鞣质含量.结果:没食子酸在0.306～2.448 mg·L-1与吸光度呈良好线性关系(r=0.999 1),平均加样回收率99.27％,RSD 2.30％.不同产地余甘子药材总鞣质含量差别较大(6.72％ ～ 11.95％),印度和尼泊尔产余甘子中总鞣质含量最高.结论:该方法简单、准确、重复性好,可用于测定余甘子中总鞣质含量.

Determination of Total Tannins in Fruits of Tibetan Medicine Phyllanthus emblica from Different Areas

Objective: To develop a phosphomolybdic acid-casein spectrophotometry for determination of total tannins in fruits of Phyllanthus emblica from different areas. Method: With gallic acid as reference substance and phosphomolybdic acid as chromogenic agent,detection wavelength 760 nm,the content of total tannins in P. emblica from eight different areas was determined. Result: The linearity range of gallic acid was 0.306-2.448 mg·L^-1 with r=0.999 1,the average recovery was 99.27% with RSD 2.30%.The content of total tannins in P. emblica from different areas were significantly different (6.72%-11.95%),the content of total tannins in samples from India and Nepal were the highest. Conclusion: This method was simple,accurate and reproducible which could be adopted for determination of total tannins in P. emblica.