葛根素、齐墩果酸及其配伍对T2DM大鼠氧化应激和炎症反应的影响

目的:观察葛根素、齐墩果酸及其配伍对2型糖尿病大鼠血糖、血脂、血清超氧化物歧化酶(SOD)、丙二醛(MDA)、糖基化终末产物(AGEs)、超敏C反应蛋白(hs-CRP)及胰腺病理组织学的影响.方法:以高糖、高脂饮食联合链脲佐菌素(30 mg·kg-1)ip法建立2型糖尿病大鼠模型,随机分为模型(M)组、二甲双胍(D)组(100 mg·kg-1·d-1)、葛根素(P)组(30 mg·kg-1·d-1)、齐墩果酸(OA)组(10 mg·kg-1·d-1)、葛根素+齐墩果酸(POA)组(40 mg·kg-1·d-1),同期设正常对照组(K).各治疗组分别ig给予相应药物治疗.治疗前及治疗2,4,6周分别测定各组大鼠空腹血糖(FBG)及餐后2h血糖(P2hPG),并于给药6周后,测定大鼠总胆固醇(CHOL)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),hs-CRP,血清SOD,MDA,AGEs水平,光镜下观察胰腺病理组织学变化.结果:M组大鼠FBG,P2hPG,CHOL,TG,LDL-C,hs-CRP,MDA,AGEs较K组升高,HDL-C和血清SOD水平明显下降,有统计学差异,对胰腺胰岛细胞有一定损伤.各治疗组大鼠FBG,P2hPG,CHOL,TG,LDL-C,hs-CRP,MDA,AGEs较M组降低,HDL-C和血清SOD水平增加,对胰腺胰岛细胞损伤有不同程度改善.POA组LDL-C水平较P组、OA组降低明显(P＜0.05),对胰腺胰岛细胞损伤明显改善.结论:葛根素、齐墩果酸及其配伍可改善实验性2型糖尿病大鼠的糖、脂代谢,其作用机制可能与抗氧化、抑制炎症反应有关.其中葛根素配伍齐墩果酸在降低LDL-C水平、改善胰岛细胞结构方面优于单纯葛根素、齐墩果酸.

Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus

Objective: To observe effects of puerarin and oleanolic acid on blood glucose, blood lipid, superoxide dismutase (SOD), malondialdehyde(MDA), advanced glycation end products (AGEs), serum hypersensitive C-reactive protein (hs-CRP) and pancreatic histopathology in rats with type 2 diabetes mellitus(T2DM), and to approach their pharmacological mechanism. Method: T2DM rats induced by high calories diet and intraperitoneal injection of streptozotacin(STZ) were randomly divided into five groups:diabetes mellitus (M)group, melbine(D) group, puerarin(P) group, oleanolic acid group(OA) and puerarin with oleanolic acid(POA)group. Fasting blood glucose (FBG) and 2-hour postprandial blood glucose (P2hPG) of rats in each group were measured before treatment and after 2, 4, 6 weeks of administration. After 6 weeks, blood simples were collected for measurement on blood glucose, as well as following indexes, cholesterol (CHOL), triglyceride (TG), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C), hs-CRP, SOD, MDA and AGEs. Pathological changes were observed under light microscope by HE staining. Result: All indexes were inhibited to certain degrees in every treatment group except HDL-C and SOD. The level of LDL-C in POA group decreased more significantly than that in the P group and OA group(P<0.05), meanwhile, the injury pancreas tissue was improved significantly. Conclusion: The puerarin and oleanolic acid could markedly improve glucose and lipid metabolism in experimental type 2 diabetic rats. It may prove that puerarin with oleanolic acid can relieve the degree of oxidative stress reaction through antioxidant activity and suppressing inflammatory reaction. In terms of decreased LDL-C and improvement of the structure of islet cells, the effects in POA group are more stronger than those in P and OA group.