日服用次数对大黄体外抗氧化效应的影响

目的:研究日服用次数对大黄含药血清体外抗氧化效应的影响,制定大黄用于抗氧化的合理日服用次数.方法:Wistar大鼠日服1次组(G1),2次组(G2),3次组(G3),分别每日灌服大黄提取物3.6 g·kg-11次、1.8 g·kg-12次、1.2g·kg-13次,分别于第1次给药前(0 h)及给药后0.083,0.167,0.25,0.5,1,2,4,6,8,10,12,24 h眼眶后静脉取血,用于测定大黄含药血清体外对超氧阴离子自由基(O2+)、二苯代苦味酰基自由基(DPPH)以及羟基自由基的清除抗氧化实验,并对其进行房室模型的拟合和参数计算,得到抗氧化效应-时间曲线(E-T曲线),制定大黄用于抗氧化时合理的日服用次数.结果:日服用次数与大黄抗氧化作用的强弱显著相关,给药2次E-T曲线下面积(AUCE)明显高于给药1次和3次(P＜0.05).结论:大黄用于抗氧化日服用次数以每日2次为宜.

Impact of Daily Administration Frequency on Antioxidant Effect in vitro of Rhubarb

Objective: To investigate the influence of daily administration frequency of rhubarb on the antioxidant effect in vitro and to determine the rational frequency for rhubarb application. Method: Rhubarb extract was administrated to Wistar rats(G1,G2 and G3 group)in a dose of 3.6 g·kg^-1 once a day, 1.8 g·kg^-1 twice a day, 1.2 g·kg^-1 three times a day, respectively. Blood sample was collected from orbital venous before the administration (0 h) and after the administration of 0.083,0.167,0.25,0.5,1,2,4,6,8,10,12,24 h. Blood sample was used for the experiments of antioxidant in vitro, such as scavenging effect of superoxide anion radical, diphenyl-generation bitterness acyl radical and hydroxyl radicals. And then antioxidant effect-time curve was obtained by fitting its compartment model and calculating parameters. Result: There was good correlation between daily administration frequency and antioxidant effects of rhubarb. Area under the curve of E-T (AUC_E) of administration twice daily was significantly higher than those of administrated 1 or 3 times daily (P<0.05). Conclusion: It is more appropriate to administrate rhubarb twice daily when it is used as antioxidant.