黄体生成素类似物对离体大鼠颌下腺细胞分泌神经生长因子的影响

目的:探讨黄体生成素(LH)类似物对离体大鼠颌下腺细胞分泌神经生长因子(NGF)的影响.方法:体外孵育大鼠颌下腺细胞并给予不同浓度的LH类似物,采用酶联免疫分析(ELISA)方法检测上清液中神经生长因子的含量.结果:当加入终浓度为10-6、10-4、10-2U/L的LH类似物孵育颌下腺细胞时,NGF的分泌量随着浓度的升高而逐渐增高;当LH类似物浓度为10-2、100、102 U/L时,NGF的分泌量随LH类似物浓度的递减而降低;同时随着时间的增加,NGF的分泌量也会增加,孵育8 h时达高峰,随后开始下降.结论:在体外LH类似物可双项调节大鼠颌下腺细胞分泌NGF的功能.

Effects of LH analogs on the secretion of NGF in submaxillary gland cells of rats in vitro

AIM: To investigate the effects of luteinizing hormone (LH) analogs on the secretion of nerve growth factor (NGF) in submandibular gland cells of rats in vitro. METHODS: The submandibular gland cells of rats were incubated in vitro and LH analogs at various concentration were added to the medium. The never growth factor (NGF) was detected by enzyme-link immunoassay (ELISA). RESULTS: When LH analogs were at the concentration of 10(-6), 10(-4), 10(-2) U/L respectively, the secretion of NGF was gradually increased. However, when LH analogs were at the concentration of 10(-2), 10(0), 10(2) U/L respectively, the secretion of NGF was gradually decreased. When LH analogs (10(-5) IU/mL) were incubated at different time, the secretion of NGF was different. NGF reached the peak value when it was incubated for 8 h and then decreased gradually. CONCLUSION: LH analogs may regulate the secretion of NGF in the submaxillary gland cells of rats.