封闭枯否细胞对肠缺血-再灌注小鼠肝细胞凋亡的影响

目的：观察封闭枯否细胞前后小鼠肠缺血－再灌注时肝细胞凋亡的变化，探讨枯否细胞在创伤后全身炎症反应综合征（ＳＩＲＳ）或多器官功能失常综合征（ＭＯＤＳ）中的可能作用。方法：用三氯化钆（ＧｄＣｌ３）特异性地封闭枯否细胞吞噬功能后，复制小鼠肠缺血－再灌注模型，动物分对照组和实验组。用原位末端标记（ＴＵＮＥＬ）法和流式细胞仪检测缺血前、缺血后６０分钟、再灌注３０分钟和再灌注６０分钟肝细胞凋亡。结果：肝细胞凋亡随肠缺血－再灌注时间延长而增多；封闭枯否细胞后，肝细胞凋亡显著增加。结论：枯否细胞功能状态对肠缺血－再灌注早期肝细胞凋亡有影响，枯否细胞功能不全可能与创伤后ＳＩＲＳ或ＭＯＤＳ发生有关。

Effects of Kupffer cell blockade on hepatocyte apoptosis after intestinal ischemiareperfusion in mice

Objective:To determine the potential role of Kupffer cells in systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS),and investigate apoptosis of hepatocytes following early intestinal ischemiareperfusion (IIR).Methods:Kupffer cells of BALB/c mice were blocked or unblocked by injecting 27 ml/kg of 0 26 g/L GdCl 3 or equal volume of 0 9% NaCl via tail vein.Fortyeight hours later,the superior mesenteric artery was occluded for 60 minutes then declamped to reproduce an intestinal ischemiareperfusion model.TdTmediated dUTP nick end labeling(TUNEL) and flow cytometry were used to detect apoptosis of hepatocytes.Results:Apoptosis of hepatocytes was increased at 60 minutes after ischemia,and 30 as well as 60 minutes after reperfusion.It was even more significantly increased after Kupffer cell blockage with GdCl 3.Conclusions:The occurrence of apoptosis of hepatocytes following early IIR may be related with Kupffer cells.The functional state of Kupffer cells may be associated with SIRS/MODS after trauma.