High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat. |
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| Authors: | T Sakai A Habiro S Kawahara |
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| Affiliation: | Faculty of Agriculture, Miyazaki University, Japan. a0b102u@cc.miyazaki-u.ac.jp |
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| Abstract: | The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol. |
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