| bFGF聚乳酸纳米微球对兔成骨细胞增殖的影响 |
| |
| 引用本文： | 张纲,谭颖徽,卢来春,何飞,裘松波.bFGF聚乳酸纳米微球对兔成骨细胞增殖的影响[J].口腔医学研究,2007,23(1):39-41. |
| |
| 作者姓名： | 张纲 谭颖徽 卢来春 何飞 裘松波 |
| |
| 作者单位： | 第三军医大学新桥医院口腔科,重庆,400037;第三军医大学新桥医院药剂科,重庆,400037 |
| |
| 基金项目： | 第三军医大学校管课题(编号:2004D107)，全军医药卫生科研基金资助项目(编号:01MA165) |
| |
| 摘 要： | 目的：观察碱性成纤维生长因子聚乳酸纳米微球缓释系统（bFGF—PLA—Ns）对体外培养的兔成骨细胞的增殖影响。方法：体外培养兔成骨细胞并鉴定，将bFGF—PLA—Ns和成骨细胞一起培养，采用MTr法检测微球对成骨细胞增殖状况的影响，免疫组化检测增殖细胞核抗原（PCNA）在成骨细胞中表达，并与单纯bFGF的作用进行比较。结果：bFGF—PLA—Ns具有良好的生物活性，能显著促进兔成骨细胞的增殖，其效应高于单纯施加bFGF的效应。结论：制备的bFGF—PLA—Ns比单纯的bFGF有更为显著的生物学效应，在骨创伤的治疗中有良好的前景。
|
| 关 键 词： | 碱性成纤维生长因子 微球 缓释 增殖 成骨细胞 |
| 文章编号： | 1671-7651(2007)01-0039-03 |
| 修稿时间： | 2006年9月8日 |
Effect of Basic Fibroblast Growth Factor-Polylactide Sustained Release Nanospheres on Proliferation of Rabbit Osteoblast in Vitro |
| |
| ZHANG Gang,TAN Yin-hui,LU Lai-chun,et al..Effect of Basic Fibroblast Growth Factor-Polylactide Sustained Release Nanospheres on Proliferation of Rabbit Osteoblast in Vitro[J].Journal of Oral Science Research,2007,23(1):39-41. |
| |
| Authors: | ZHANG Gang TAN Yin-hui LU Lai-chun |
| |
| Institution: | ZHANG Gang,TAN Yin-hui,LU Lai-chun,et al.Department of Stomatology,Xin Qiao Hospital,the Third Military Medical University,Chongqing 400037 |
| |
| Abstract: | Objective:To evaluate the proliferative effect of basic fibroblast growth factor-polylactide sustained release nanospheres(bFGF-PLA-Ns) on cultured rabbit osteoblast in vitro.Methods: Cell culture technique and MTT colorimetric assay were used to evaluate the proliferation of rabbit osteoblast by adding bFGF-PLA-Ns or only bFGF.Expression of proliferating cell nuclear antigen(PCNA) was examined with immunohistochemical staining.Results: The results showed that bFGF-PLA-Ns had biological activity.Statistical analysis showed that bFGF-PLA-Ns could enhance significantly more proliferation of rabbit osteoblast than that of bFGF.Conclusion: bFGF-PLA-MS enhances proliferation better than bFGF on rabbit osteoblast. |
| |
| Keywords: | Basic fibroblast growth factor Nanospheres Sustained release Proliferation Osteoblast |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
