胃癌患者血清中可溶性CD44v6含量和组织中CD44v6蛋白表达的研究

目的:研究胃癌患者外周血清中可溶性CD44v6(sCD44v6)含量/CD44v6蛋白表达,及其与临床病理参数之间的关系。方法:应用定量ELISA方法检测胃癌患者(术前和术后)及健康对照组血清中sCD44v6含量,并以(S-P)免疫组化法测定相应组织中CD44v6蛋白的表达。结果:70例胃癌患者血清中sCD44v6含量(2.15±0.78 ng/ml)明显高于16例正常对照组(1.18±0.43 ng/ml),14树根治性手术后血清中sCD44v6含量(1.21±0.39 ng/ml)比术前(2.67±0.83 ng/ml)明显下降(P<0.01),而6例非根治性手术后(3.29±0.41ng/ml)比术前(3.61+0.49 ng/ml)下降不明显(P>0.05)。而胃癌组织中CD44v6蛋白表达在肠型和弥漫型中有显著差异,不同的浸润深度、有无淋巴结及远处转移也有显著差异。结论:胃癌患者外周血清中sCD44v6含量及组织中CD44v6蛋白表达的变化与转移、临床分期、病理分期有关,sCD44v6含量升高可作为胃癌患者淋巴结转移(尤其是早期转移)的监测指标。

Serum CD44 Variant 6 as an Indicator of Tumor Burden and Metastasis in Patients with Gastric Carcinoma

Objective: To study the expression of CD44 correlation with the ability of metastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration of soluble CD44 Variant 6 (sCD44v6) and the histolagic expression of CD44 Variant 6 (CD44v6) in tumors with the clinico-pathologic features, and to make serum concentration of t/te CD44v6 and the expression of CD44v6 may be useful an indicator as an early diagnosis, invasion, metastasis, and prognosis. Methods : Serum samples were obtained from 7 0 patients with primary gastric carcinoma before surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with a quantitative-enzyme-link-immuno-adsorbent assay. The expression of CD44v6 in tumors was examined by immuno-histo-chemical staining. Results. Both the serum concentration of CD44v6 and its expression in tumors were positively related to the depth of invasion of the tumor, lymph node metastasis, clinical stage, and diffuse type gastric carcinoma, but not to tlie tumor size. The serum level of CD44v6 in patients with gastric cancer was significantly higher than that in control. The serum concentration of CD44v6 was markedly lowered after curative surgery (P<0. 001), and tlie serum level of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than in those with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator in patients with diffuse type gastric carcinoma, as was the histohgical expression of CD44v6. Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to the progression of diffuse type gastric carcinoma metas- tasis and clinical stage. An elevated level of sCD44v6 may serve as an indicator of lymph node metastasis (especially early metastasis) and bad prognosis in patients with gastric carcinoma.