3种藏药秦艽花脂溶性成分的GC-MS分析

目的： 分析西藏秦艽花，麻花秦艽花和川西秦艽花脂溶性的成分。方法： 采用索氏提取法获得脂溶性成分，经GC-MS技术结合计算机检索对其化学成分进行分离和鉴定，用色谱峰面积归一化法计算各个组分峰的相对含量。结果： 从西藏秦艽花，麻花秦艽花和川西秦艽花的脂溶性成分中分别鉴定了38，35，27个化合物，占其各自总量的94.31%，83.09%，80.84%。3种秦艽花脂溶性成分存在一定程度的差异，西藏秦艽花的主要成分为饱和烷烃（59.33%）、醇类（14.53%）、有机酸（6.43%）、酮类（3.84%），麻花秦艽花的主要成分为饱和烷烃（28.24%）、醇类（17.51%）、有机酸（8.09%）、酯类（7.55%），川西秦艽花的主要成分为饱和烷烃（24.79%）、醇类（5.79%）、生物碱（17.03%）、醌类（16.60%）、酯类（2.78%）。其中川西秦艽花的脂溶性成分跟其他2种的区别较大，预示其药理活性的不同。结论： 通过对其脂溶性成分和含量的分析评价与比较， 为开发利用秦艽花资源提供科学依据。

Analysis on Fat-soluble Constituents in Flowers of Tibetan Medicinal Plants of Three Gentiana Species by GC-MS

Objective: To analyze fat-soluble constituents in flowers of Tibetan medicinal plants of three Gentiana species, G. dendrologi, G. straminea and G. tibetica. Method: The fat-soluble components were extracted by Soxhlet extraction, separated and identified using GC-MS. The relative content of each component was determined by area normalization. Result: Thrity-eight, thrity-five and twenty-seven compounds from G. dendrologi, G. straminea and G. tibetica were identified respectively. The ratios of identification of total compounds were 94.31%, 83.09% and 80.84% respectively. There are some differences among three Gentiana, the main ingredients being alkane (59.33%), ethanol (14.53%), and organic acid (6.43%) and ketones (3.84%) in flowers of G. dendrologi, alkane (28.24%), ethanol (17.51%), and organic acid (8.09%) and esters (7.55%) in G. straminea, and alkane (24.79%), ethanol (5.79%), and alkaloids (17.03%), quinones (16.60%) and esters (2.78%) in G. tibetica G. tibetica showed different pharmacological activity owing to different chemical compositions. Conclusion: By analyzing and evaluating their fat-soluble components in three Gentiana Species, the scientific data can be provided for exploitation of Gentiana resources.