| 高效液相色谱法测定残黄片中盐酸小檗碱的含量 |
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| 引用本文： | 张诗龙,刘峰群,周旭,杜凤霞,吴素体,王伊文,陈红鸽.高效液相色谱法测定残黄片中盐酸小檗碱的含量[J].华北国防医药,2011,23(3):47-48. |
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| 作者姓名： | 张诗龙 刘峰群 周旭 杜凤霞 吴素体 王伊文 陈红鸽 |
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| 作者单位： | 解放军302医院药学部,北京,100039 |
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| 摘 要： | 目的 建立残黄片中盐酸小檗碱含量测定方法.方法 采用高效液相色谱法,色谱柱:Luna C18 (5 μm,250 mm×4.6 mm)反相柱;乙腈-0.05 mol/L磷酸二氢钾溶液(50∶50)(每100 ml中加十二烷基硫酸钠0.4 g,再以磷酸调节pH值为4.0),用前经0.45 μm滤膜滤过;流速1.0 ml/...
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| 关 键 词： | 盐酸小檗碱 残黄片 色谱法 高效液相 质量控制 |
Content Determination of Berberine Hydrochloride in Canghuang Tablet by High Performance Liquid Chromatography |
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| ZHANG Shi-long,LIU Feng-qun,ZHOU Xu,DU Feng-xia,WU Su-ti,WANG Yi-wen,CHEN Hong-ge.Content Determination of Berberine Hydrochloride in Canghuang Tablet by High Performance Liquid Chromatography[J].Medical Journal of Beijing Military Region,2011,23(3):47-48. |
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| Authors: | ZHANG Shi-long LIU Feng-qun ZHOU Xu DU Feng-xia WU Su-ti WANG Yi-wen CHEN Hong-ge |
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| Institution: | (Department of Pharmacy,the 302nd Hospital of PLA,Beijing 100039,China) |
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| Abstract: | Objective To establish a method for determining berberine hydrochloride in Canhuang Tablet.Methods The high performance liquid chromatography was used for determining the content of berberine hydrochloride.The reversed-phase column was a Luna C18（5 μm,250 mm×4.6 mm）.Acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution（50∶50,0.4 g sodium dodecylsulfate/100 ml,with a pH value of 4.0 adjusted by phosphoric acid） was filtered through 0.45 μm filtration membrane before administration.The detective wavelength was set at 345 nm with a flow rate of 1.0 ml/min,and column temperature at 30℃.Results Berberine hydrochloride was acquired in the range of 60.54～141.26 μg/ml（r=0.9999）with good linear correlation.The average recovery rate and RSD were 101.09% and 2.02% respectively（n=6）.Conclusion The method is accurate and reliable in determining berberine hydrochloride in Canhuang Tablets. |
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| Keywords: | Berberine hydrochloride Canhuang tablets Chromatography high efficiency liquid Quality control |
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