| 快速冷冻切片制备方法的改进及结果观察 |
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| 引用本文： | 牛晓东,李艳霞.快速冷冻切片制备方法的改进及结果观察[J].华北国防医药,2011,23(5):4-5,I0002. |
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| 作者姓名： | 牛晓东 李艳霞 |
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| 作者单位： | 张家口,解放军251医院病理科,河北,075000 |
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| 摘 要： | 目的 探讨快速冷冻切片技术改进方法在病理诊断中的应用价值.方法 对我院2010年手术切除的乳腺、甲状腺、子宫、喉、脑组织等600例新鲜组织标本,采用医用耦合剂包埋,恒温式冰冻切片机切片,苏木素加热伊红快速染色后封片观察.结果 此方法制片时间约8 min,镜下组织结构和组织切面完整,细胞形态清晰,染色佳,组织无冰晶形成及...
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| 关 键 词： | 冷冻切片 组织包埋 染色与标记 |
Improvement and Observation of Quick Frozen Sections Preparation |
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| NIU Xiao-dong,LI Yan-xia.Improvement and Observation of Quick Frozen Sections Preparation[J].Medical Journal of Beijing Military Region,2011,23(5):4-5,I0002. |
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| Authors: | NIU Xiao-dong LI Yan-xia |
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| Institution: | NIU Xiao-dong,LI Yan-xia(Department of Pathology,the 251st Hospital of PLA,Zhangjiakou,Hebei 075000,China) |
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| Abstract: | Objective To discuss the application value of improvement methods of quick frozen sections preparation in the pathological diagnosis.Methods 600 tissues of mammary glands,thyroid glan,uterus,larynx and brain selected in surgery during 2010 were observed using medical couping agent,constant temperature type frozen section machine slice,fast staining of heating haematoxylin and eosin and sealing.Results The production time was 8 min,microscopical organization structure and slice organization were complete,cel... |
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| Keywords: | Frozen section Tissue embedding Staining and labeling |
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