声带息肉的电镜观察

目的 探讨声带息肉病变组织中成纤维细胞、胶原纤维、弹性纤维超微结构改变.方法对手术切除的10例声带息肉和3例正常声带对照(下咽癌行喉全切除见声带未受肿瘤侵犯)行透射和扫描电镜观察.透射电镜采用常规方法;扫描电镜采用组织消化法,观察弹性纤维的标本用90%甲酸消化,观察胶原纤维的标本用10%NaOH水溶液消化.结果 透射电镜下声带息肉固有层中成纤维细胞数量增多,细胞器丰富,可见大量内质网、高尔基复合体、线粒体,表明成纤维细胞处于功能活跃状态.固有层内可见炎性细胞浸润,提示炎症反应与发病有关.声带息肉中血管数量明显增多.扫描电镜观察,声带息肉中胶原纤维和弹性纤维的形态发生改变,纤维排列紊乱.结论 声带息肉中成纤维细胞、弹性纤维、胶原纤维形态改变,这些变化可能是影响声带发声功能的病理学基础.

Electron microscopic observation of vocal fold polyps

OBJECTIVE: The purpose of this study was to observe the ultrastructure of the fibroblasts, collagen and elastic fibers in vocal fold poiyps. METHODS: Ten vocal fold polyps and 3 normal vocal fold specimens obtained from total laryngectomy were studied by means of transmission electron microscope and scanning electron microscope. RESULTS: The result showed that in vocal fold polyps, the quantity of fibroblasts increased and there were abundant organelles, suggesting that the fibroblast were in the status of activation. As the main cell to produce lamina propria extracellular matrix, the representation suggested that the extracellular matrix metabolism was active. Leucocytes soakage was observed, suggesting that the inflammation may play a role in the lesion. It was found by scanning electron microscopy that in case of lesions, collagen fibers and elastic fibers arrayed irregularly. CONCLUSIONS: Under pathologic circumstance, fibrobalsts, collagen and elastic fibers altered in morphology, which possibly induced the functional alteration.