脂质体介导Bcl-2反义寡核苷酸对胆管癌细胞株QBC939的作用

目的：观察Bcl-2反义硫代磷酸寡脱氧核苷酸（ASODN）对人胆管癌QBC939细胞的作用及对Bcl-2蛋白表达的影响。方法：脂质体LipofectamineTM2000介导Bcl-2 ASODN转染QBC939细胞，应用台盼篮拒染实验检测细胞存活率、克隆形成实验检测克隆形成率、Western blot检测Bcl-2蛋白表达。结果：Bcl-2 ASODN转染后Bcl-2蛋白免疫印迹条带光密度显著低于对照组（P<0.01）；台盼篮拒染实验和克隆形成实验均显示Bcl-2 ASODN可以部分抑制QBC939细胞增殖，经ASODN作用，细胞的存活率和克隆形成率均显著低于对照组（P<0.05）。结论：Bcl-2 ASODN通过封闭人胆管癌QBC939细胞bcl-2基因表达抑制人胆管癌QBC939细胞的增殖。

Effect of Bcl-2 antisense phosphorothioate oligodeoxynucleotides on bile duct carcinoma cell line QBC939

Objective To investigate the effect of Bcl-2 antisense oligodeoxynucleotides (ASODN) on the cell proliferation and Bcl-2 expression in bile duct carcinoma cell line QBC939. Methods Bcl-2 ASODN and control sequence were transfected into cell line QBC939 by Lipofectamine~TM2000.The changes of Bcl-2 protein were detected by Western blot. The survival rate and colony formation rate of QBC939 cells incubating with Bcl-2 ASODN were evaluated by trypan blue staining assay and colony forming test. Results The densitometric analysis of Gel-photograph showed that the level of Bcl-2 protein expression in the ASODN transfected group was significantly lower than that in the controls (P<0.01). Both trypan blue staining assay and colony forming test demonstrated that Bcl-2 ASODN could partially inhibit the growth of QBC939 cells. After incubating with Bcl-2 ASODN, the survival rate and colony formation rate of QBC939 cells were significantly lower than those of the controls (P<0.05). Conclusion Bcl-2 ASODN inhibits the cell proliferation in bile duct carcinoma cell line QBC939 by blocking the expression of bcl-2 gene.