酚氨咖敏颗粒含量测定方法的改进

目的：建立HPLC法同时测定酚氨咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量。方法：色谱柱：Kromasil C18（250mm×4．6mm，5μm）；流动相：1％醋酸溶液（用三乙胺调pH为3．5）-甲醇-乙腈（65：10：25）；流速：1．0ml·min^-1；检测波长：273nm；柱温：30℃。结果：对乙酰氨基酚的线性范围为12．3～123．2μg·ml^-1（r=0．9996），平均回收率为98．65％，R．如为0．31％（n=5）；咖啡因的线性范围为2．44～24．42μg·ml^-1（r=0．9998），平均回收率为99．15％，RSD为0．15％（n：5）；氨基比林的线性范围为8．23～82．34μg·ml^-1（r=0．9999），平均回收率为98．40％，RSD为0．39％（n=5）。结论：方法简单可行，可用于酚氨咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量测定。

Improvement of Determination Method of Paracetamol, Aminophenazone Phenacetin, Caffeine and Chlorphenamine Maleate Granules

Objective： To establish an ttPLC method for determination of paracetamol, caffeine, aminopyrine in paracetamol, aminophenazone phenacetin,eaffeine and chlorphenamine maleate granules simultaneously. Method： The Kromasil C18 column （250 mm×4. 6 mm , 5 μm） was used with a mixture of 1% acetic acid solution （adjust pH to 3. 5 by triethylamine）-methanol-acetonitrile （65： 10：25 ） as the mobile phase. The detection wavelength was at 273 nm , the flow rate was 1.0 ml.min ^-1, and the temperature of the column was 30 ℃. Result： The linear range was 12. 3-123.2μg. ml^-1 （r = 0. 999 6） for paracetamol and the average recovery was 98.65% with RSD of 0. 31% （n =5）, the linear range was 2. 44-24. 42 μg.ml^-1 （r =0. 999 8） for caffeine and the average recovery was 99. 15% with RSD of 0. 15% （n =5） , and the linear range was 8. 23-82. 34 μg.ml^-1 （r =0. 999 9） for aminopyrine and the average recovery was 98. 40% with RSD of 0. 39% （ n = 5 ）. Conclusion ： The method is simple and can be used for the determination of paracetamol, caffeine, aminopyrine in paracetamol, aminophenazone phenacetin, caffeine and chlorphenamine maleate granules.