正交试验优选柿蒂鞣质的提取工艺

目的：优选柿蒂中鞣质类成分的提取工艺。方法：运用正交设计方案，采用磷钼钨酸-干酪素比色法，以没食子酸为对照考察了各种提取因素对提取率的影响，优化冷浸法和回流法的工艺条件。结果：两种提取方法中，回流提取效果较冷浸提取好。最佳提取工艺条件是溶剂为70％的丙酮，料液比为1：15，回流时间1．5h，回流温度40℃。柿蒂鞣质含量约为2．04％。结论：优化出的最佳提取工艺条件是合理的，鞣质成分提取率高。

Optimization of Extracting Technology of Tannins in Persimmon Calyx by Orthogonal Design

Objective： To optimize the technology of extraction of tannin in Persimmon Calyx. Method ： The orthogonal design was performed, a modified tungsten molybdophosphate-casein colorimetric method was used with gallic acid as reference substance to test the main factors, the extracting conditions of cold soaking and refluxing extraction was optimized. Result： Compared with the cold soaking method, refluxing extration was more reliable with higher extratcting ratio. The optimum extraction process conditions were as follows ： adding 70% acetone, refluxing 1.5 hours at 40 ℃. The total tannin contained in the Persimmon Calyx was about 2. 04%. Conclusion： The optimum conditions of extraction process were reliable and the extration ratio of tannin was high.