丹参对体外高糖环境中大鼠系膜细胞活性氧抑制作用的研究

目的：观察丹参对高糖环境中系膜细胞所产生的活性氧（ROS）及转化生长因子β1（TGF—β1）的抑制作用。方法：将细胞分为以下6组：对照组（C组，普通MEM培养基，含5．6mmol·L^-1葡萄糖）；高糖组（H组，30mmol·L^-1高糖MEM培养基）；小剂量丹参干预组（S1组，H组＋37．5mg·ml^-1丹参）；中剂量丹参干预组（S2组，H组＋75mg·ml^-1丹参）；大剂量丹参干预组（S3组，H组＋150mg·ml^-1丹参）；单纯丹参组（S组，C组＋150mg·ml^-1丹参）。分别采用激光共聚焦和ELISA法检测ROS及TGF—β1水平。结果：H组ROS水平较C组明显增高，分别采用不同浓度丹参干预后，ROS水平均显著降低，并呈剂量依赖性；与H组相比，不同剂量丹参干预组TGF-β1表达均降低，但仅S3组TGF—β1的下降具有统计学意义。与对照组相比，单纯丹参干预组中ROS和TGF—β1水平无明显变化。结论：丹参可减少高糖环境中系膜细胞ROS及TGF—β1的产生，而这可能是丹参发挥肾脏保护作用机制之一。

Inhibitory Effects of Salvia miltiorrhiza on the Expression of Reactive Oxygen Species in Rat Mesangial Cells Cultured in High Glucose

Objective： To investigate the inhibitory effects of Salvia miltiorrhiza on the expression ot reactave oxygen species （ ROS） and transforming growth factor-β1 （TGF-β1 ） induced by high glucose in rat mesangial cells. Method： The mesangial cells were divided six groups ： control group （ C, 5.6 mmol ·L - 1 glucose） ; high glucose group （ H, 30 mmol· L - 1 glucose ） ; S1 group （ S1, group H ＋ 37. 5 mg·ml - 1 Salvia miltiorrhiza ） ; S2 group （ S2, group H ＋ 75 mg·ml - 1 Salvia miltiorrhiza） ; S3 group （ S3, group H ＋ 150 Mg· ml - Salvia mihiorrhiza） ;S group （ S, group C ＋ 150 mg·ml -1 Salvia miltiorrhiza）. The levels of ROS and TGF-β1 were measured by eonfocal laser scanning microscopy and ELISA respectively. Result： The levels of intraeellular ROS in group H were higher than that in group C ,which were prevented by Salvia mihiorrhiza. And there were dose-dependent. Comparing with group H, TGF-β1 expressions were decreased in three group treated with Salvia miltiorrhiza. Only the decrease of TGF-β1 in group S3 was significant. Comparing with group C ,the levels of ROS and TGF-β1 in group S were not change. Conclusion： Salvia mihiorrhiza may have protective effects on kidney by suppressing the over-expressions of ROS and TGF-β1 in rat mesangial cells induced by high glucose.