抗复感颗粒对脾虚小鼠抗应激和急性毒性实验研究

目的：研究抗复感颗粒对脾气虚症小鼠抗应激反应的能力和急性毒性。方法：给小鼠皮下注射利血平制成脾虚模型，灌胃给予复感颗粒（12．14，9．10，6．83g生药／kg），观察抗复感颗粒对脾虚小鼠体重、体温、带负荷游泳时间、耐低温能力的影响；观察抗复感颗粒对小鼠经灌胃给药的急性毒性。结果：抗复感颗粒能显著提高脾虚小鼠的体温和体重，能显著延长小鼠负重游泳时间及提高耐寒能力（P〈0．05，P〈0．01）；小鼠灌服抗复感颗粒的急性毒性实验未观察到明显毒性反应，最大给药量为267．48g生药／kg。结论：抗复感粒颗可改善脾虚小鼠的体温和体重，具有抗疲劳、耐寒作用，可增加小鼠抗应激反应的能力，且具有良好的安全性。

Study of Kangfugan Granules on Anti-stress Activity and Acute Toxicity in Mice with Spleen Deficiency Syndrome

Objective ： To study the effect of kangfugan granules （GFGG） on anti-stress response of mice with spleen deficiency syndrome （SDS） and acute toxicity in mice. Method： The experimental model of mice with SDS was induced by reserpine. Then the mice were treated with three dosages of GFGG （ 12. 14,9. 10,6. 83 g·kg^-1 ）, respectively. The swimming time was observed with loaded swimming and the survival time was observed with low temperature. Result： The time of loaded swimming was extended and ability of cold resistance was improved by GFGG,obvious toxic reaction was not observed and the maximum tolerant dose was 267.48 g-kg-1 for mice in acute toxicity experiment. Conclusion： GFGG has obvious effects of anti-fatigue and low- temperature-resistance,increased the ability of stress response in mice, and it is favorable safe.